Aims To understand the Staphylococcus coagulans prevalence in causing skin infections in dogs and detection of various virulence genes in Staph. coagulans isolates. Methods and Results Staph. coagulans was isolated from pus swabs collected from dogs with skin infection and identified by detecting thermonuclease, coagulase, and urease genes. The presence of methicillin-resistant gene (mecA) was performed by PCR. Antimicrobial susceptibility test was carried out by disc diffusion method. In total, 38 Staph. coagulans clinical isolates and 42 Staph. coagulans genomes available in NCBI database were screened for 19 virulence genes by PCR and in silico prediction, respectively. A prevalence of 13.8% (38/275) of Staph. coagulans dog skin infection was observed and 15.8% (6/38) of Staph. coagulans isolates carried mecA gene. Many Staph. coagulans isolates were susceptible to all tested antimicrobials. Twenty nine per cent isolates were resistant to ciprofloxacin. Genes encoding leukotoxins, DNase, exfoliative toxin, superantigen-like exotoxin, immunoglobulin-binding proteins, fibrinogen-binding proteins, autolysin, and rod shape-determining protein were detected in almost all the Staph. coagulans clinical isolates and genomes from NCBI database, whereas anti-adhesin plasma-sensitive protein genes were present in relatively lesser number of Staph. coagulans clinical isolates and genomes from NCBI database. Conclusions Staph. coagulans possesses many virulence factors that are present in other coagulase-positive staphylococci, such as Staph. aureus and Staph. pseudintermedius. The presence of two bi-component leukotoxin genes in tandem with other virulence factor genes in a single pathogenic island in the Staph. coagulans genomes explained their eminence in the virulence of Staph. coagulans causing infections. Staph. coagulans was classified as a separate species in the year 2020 and primarily causes skin infections in dogs. Identification of this species is not included in any of the automated bacterial identification systems. Hence, many veterinary laboratories do not have a strategy to identify this bacterium. This study will help in the identification of Staph. coagulans in veterinary laboratories by PCR apart from detecting various virulence factors present in this pathogen. The existence of many virulence factors and prevalence in different animals in varied geographical locations suggest that Staph. coagulans is an important coagulase-positive staphylococcal pathogen in animals.
Background: In India, most of the farmers are shifting towards organized dairy cattle farming system from paddy cultivation and extensive system of rearing due to scarcity of water resources and intense variance in climatic conditions. Hence, the present study provided valuable information about the existing dairy cattle housing in Cauvery delta region of Tamil Nadu. Methods: The study on existing management practices of dairy cattle was conducted randomly among 90 farmers of Cauvery delta region, Tamil Nadu. A structured interview schedule was developed and pre-tested and the data on existing management practices were collected with the help of a pre-tested interview schedule. The data were collected by personal interview of the respondents individually at their animal house. The information on dairy production system and management practices were collected and the micro climate of different dairy cattle shed were recorded and analyzed. Result: In Cauvery delta region, the majority of farmers (48.89%) engaged in rearing crossbred dairy cattle were in the middle age group and educated. Further, animal husbandry is either the primary (25.56%) or secondary (74.44%) profession of the farmers. From the study, it is understood that more than 70 per cent of farmers housed their dairy cattle in loose type of housing, adjacent to their home with east-west orientation. The most common roofing pattern adopted for their cattle sheds is gable type (54.45%), with a roofing material of galvanized iron sheet (30.00%). The black globe humidity index (BGHI) and heat load index (HLI) calculated for dairy cattle shed with different roofing structures showed significant difference (P less than 0.05) between thatched and tiled shed.
Aim: In arid and semi-arid areas recycling of water may have a greater impact on future usable water supply than any of the other technologies aimed for increasing water supply. Efforts have been taken on maximizing the benefit and minimizing the detrimental effects on people or the environment due to continuous usage of treated wastewater. Hence, the current experiment was aimed to study the effect of treated wastewater in combination with organic and inorganic nutrients on bajra napier hybrid grass on yield and quality parameters. Study Design: The field experiment was conducted using secondary treated wastewater, manure and inorganic nutrients in Bajra Napier hybrid grass variety CO(BN) 5 with a strip plot design and four replications. Place and duration of study: The experiment was conducted in Veterinary College and Research Institute, Orathanadu, Thanjavur, Tamil Nadu, during 2018-19 (10.62ᵒ N latitude and 79.26ᵒ East longitude). Methodology: The five ratios of irrigation water were used in horizontal factor treatments and four different nutrient combination treatments were allotted in the vertical factor. The plot was divided into 27 m2 with 2 m space between the plots. The two budded setts of bajra napier hybrid grass variety CO (BN)-5 were planted at 60 x 50 cm spacing. The flood irrigation and nutrients (basal and top-dressing) were applied as per the treatments. Results: The use of treated wastewater for irrigation combined with manure and inorganic nutrients significantly increased the total green and dry fodder yields. Irrigation treatment with treated wastewater alone + 100% inorganic nutrients (I5N1) recorded significantly higher total green and dry fodder yields of 431.0 and 76.7 t ha-1 yr-1, respectively, than all other treatment combinations. Irrigated with groundwater alone (control) without nutrients (I1N4) recorded the lowest total green and dry fodder yields (222.5 and 47.6 t ha−1 yr-1, respectively). The average crude protein content of all total cuttings was significantly higher (13.53%) in I5N1 than all other treatments. On the other hand, the average crude fibre content (28.03%) was significantly lower in the treatment I5N1 as compared to irrigated with groundwater without nutrients (31.18%). Conclusion: The perennial fodder crop Bajra Napier hybrid grass variety CO (BN) 5 cultivated under-treated wastewater combined with manure and inorganic nutrients increased the total green and dry fodder yields with beneficial proximates. The use of treated wastewater for fodder cultivation helps conserve groundwater and effectively utilize available nutrients in treated wastewater.
Background: Forages of high quality are the most valuable commodity of any livestock activity and they serve as the basis for most of the rations in a forage-based diet. The lack of good quality seedlings/seeds, notably improved varieties, is the primary reason for the slow adoption of improved forage production technologies. Traditional establishment of bajra napier forage incurs more labour costs, harvesting sett material, transporting, preparing two budded setts and carrying to the planting area. Nearly three to four tons of green leaves stem materials are wasted for planting one hectare of area. In this situation, an alternate method is required to produce high-quality seedlings in a short period. This research objective compared single node bud chip seedling in different protray cell cavities and pot mixtures. Methods: The green shade net field study was carried out between 2019 and 2020, the effect of different pro-tray sizes (50, 60 and 98 cell cavities) and potting mixture (red earth, cocopeat, farmyard manure, vermicompost and root growth promoters like phosphobacteria bio-fertilizer and indole acetic acid (IAA) was taken in different combination for the establishment of Bajra Napier hybrid variety Co (BN)-5 seedling by using single node bud chip technology. The observation was taken up to 30 days in related to seedling growth and development. The observed parameter was statistically analysed in SPSS software. Result: Among the three different protray cavities and five different pot mixtures, 60 cavity protray with red earth + cocopeat + vermicompost (2:1:1) results revealed significant differences (p greater than 0.05) in seedling growth parameters among the different protray and growing media. The maximum seedling growth parameters like germination, numbers of the leaf, plant height, leaf length, width and root length (94.4 per cent, 8.17, 39.12 cm, 38.14 cm, 2.5 cm and 30.8 cm) were observed at 30 days aged seedlings followed by red earth + cocopeat + farmyard manure (2:1:1 ratio) and treated with IAA substance in 60 cavity protray.
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