The scientific article presents the results of investigating the spinal cord`s morphology of a domestic bull (Bos Taurus). Data on the histo- and cytostructure of the spinal cord are given according to the results of histological, neurohistological, and morphometric studies. For their implementation, the selected material (spinal cord n = 8) was subjected to fixation in 10–12 % neutral formalin solution, followed by pouring into paraffin. Histological sections were made from paraffin blocks on a sliding microtome MS-2 with a thickness of not more than ten μm. Staining of sections with hematoxylin and eosin, according to Van Gieson's methods, as well as neuro-histological methods of impregnation of nerve tissue with silver nitrate according to the Bilshovskym-Gross method, was used for the morphometric studies, investigating the morphology of the cell, conducting and obtaining the review histological preparations. The histostructure of the spinal cord, the localization of neurons in the gray matter, and morphometric studies of structural elements were examined on histological specimens by light microscopy. The entire experimental part of the research was conducted following the requirements of the international principles of the "European Convention for the Protection of Vertebrate Animals for Experimental and Other Scientific Purposes" (Strasbourg, 1986). The spinal cord, medulla spinalis, an organ of the central nervous system of vertebrates, is located in the spinal canal. The spinal cord is protected externally by soft, arachnoid, and hard meninges. The space between the membranes and the spinal canal is filled with cerebrospinal fluid. It is well known that groups of multipolar nerve cells with the same functional value form the nuclei of the gray matter of the spinal cord. According to the results of our histological studies, a pronounced differentiation of nerve cells, which have different shapes and sizes. Among them are large, medium, and small nerve cells. The shape of nerve cells is different, which, in turn, depends on their location in certain areas of the gray matter of the spinal cord and the size of the cell. In general, there are multifaceted, stellate, spindle-shaped, elongated, rounded, and oval neurons. Small nerve cells have an oval or round, less often – irregularly rounded shape, medium – round, oval, spindle-shaped. Large nerve cells are dominated by a multifaceted shape with distinct processes. The nuclei of large nerve cells, in most cases, have a rounded shape, less often – oval, mostly in the center of the cells, seldom – eccentrically. According to the results of morphological studies, it is noted that the neurons of the gray matter of the spinal cord have different shapes and sizes. Consequently, in the gray matter, small cells are the highest quantity (47.91 ± 0.32 %) of the total number of nerve cells. The second place is occupied by average neurons (33.70 ± 0.46 %). The large cells are detected in the smallest amount (18.37 ± 0.50 %).
The study aimed to investigate the effect of the drug “Bendamine” on dogs' clinical and morphological parameters in heart failure. Two groups of dogs were formed for research: control (healthy) and experimental (sick). The 30-day experiment included ten dogs from the control group, ten dogs of the observed (unhealthy) spaniel breed, and 10–12 years old dogs. The treatment regimen for sick dogs: furosemide was administered orally on an empty stomach at 2 mg/kg every 12 hours; enalapril was administered orally at 0.5 mg/kg every 12 hours; Bendamine was administered orally on an empty stomach at 20 mg/kg every 24 hours. It is recommended to divide the dose into two doses. In the study of hematopoiesis, we found that erythrocytes in dogs with heart failure increased by 18.2 %, while hemoglobin levels decreased by 1.8 %. Before treatment, red blood cell counts were found to reduce the mean hemoglobin in the erythrocyte and the mean erythrocyte volume. In the experimental group of dogs treated with the “Bendamine”, normalization of morphological blood parameters was found. The number of erythrocytes was 5.8 ± 0.06 T/L, and the hemoglobin level was 143.5 ± 4.7 g/L. The use of the drug “Bendamine” contributed to the gradual restoration of the functional state of the hematopoietic system in dogs with heart failure. This is also indicated by the red blood cell index of the dogs after the course of treatment. The therapy application in the experimental group significantly reduced the frequency and severity of shortness of breath and increased endurance during exercise. There was also a decrease in the frequency and duration of cough attacks. After treatment, signs, and intensity of ascites decreased in dogs of the experimental group. It has been suggested that this is due to an increase in myocardial contractile function due to the positive ionotropic action of Bendamine. The owners also recorded an improvement in appetite in animals after 30 days of therapy. Even in the dogs of the experimental group, after treatment, physical activity and emotional reactions improved. They were less depressed and more inclined to communicate with the owners. Thus, the use of the cardiac drug “Bendamine” in dogs with heart failure positively affected the restoration of hematopoiesis and the normalization of morphological parameters of the blood.
The attention of modern society to euthanasia has increased because many centuries ago, a social phenomenon was established, associated not only with the achievements of medical science but also with changing values of the individual and society, actualizing the problem, considering them family members. The number of euthanasia animals has fallen sharply in recent decades. This was facilitated by the adoption of animal protection programs, as well as increasing the social value of animals – the consideration of animals not only as property but also the recognition of some of their rights to welfare. The article discusses the ethical aspects of veterinary practice related to euthanasia, as the relationship between animal and human is constantly changing, ultimately changing the veterinary profession's landscape. When carrying out any manipulations, the relationship between the animal and the owner should be considered. In addition, the rapid development of medicine leads to new opportunities for animal care and the creation of ethical and moral dilemmas regarding the possible improvement of patients' lives. Particular attention is paid to the impact of euthanasia on the morale of the veterinarian, as well as the level of preparation for this manipulation and further communication with the owners. The article presents the preconditions and indications for this procedure and the research results on the leading causes of euthanasia. Particular attention is paid to the euthanasia of laboratory and warm-blooded animals for use in biological experiments. Methods and methods of euthanasia of animals following the “European Convention for the Protection of Vertebrate Animals” and the Law of Ukraine “On Protection of Animals from Cruelty”, which are divided into “recommended”, “acceptable”, “conditionally acceptable”, “unacceptable”. The information on the list of possible drugs for euthanasia is given. The classification of euthanasia methods and the use of humane methods of ending animal life is given in the example of Canadian shelters.
The study of pharmacological studies of the veterinary medicinal product for external use in the form of ointment – “Dibutalastin Ointment” is a mandatory stage of preclinical research of the drug, which is a new development of PP “Biopharm” and LLC “DEVIE”. In a scientific experiment on laboratory animals, it is possible to assess the drug's safety for different terms of use to determine the zone of toxic action and doses that do not cause harmful effects on health. Therefore, the work aimed to carry out pharmacological studies of the drug with methyl salicylate and dimethyl sulfoxide in the form of an ointment for treating European fallow deer, deer, dogs, and cats with mastitis, arthritis, myositis, and injuries of various origins. The toxicological characteristics of the studied ointment “Dibutalastin” based on methyl salicylate and dimethyl sulfoxide were studied in an "acute" study on warm-blooded animals. The average lethal dose (LD50) for intragastric administration could not be established, as the volume exceeded the permissible level. It was established that according to the “average lethal dose when entering the stomach” indicator, the veterinary medicinal product belongs to relatively non-toxic substances (toxicity class IV). The results of pharmacological studies indicate the absence of resorptive-toxic effects, sensitizing properties, and irritating effects on the mucous membranes of the eyes and skin. Pathomorphological studies of animal organs when studying the acute toxicity of the drug showed that the developed ointment with methyl salicylate and dimethyl sulfoxide does not cause pathological changes in internal organs. From the point of view of veterinary toxicology, this drug is safe. Further studies will be the next stage of pre-registration tests aimed at studying the embryotoxic effect of “Dibutalastin Ointment”, which is mandatory material of the “Safety and residue studies” section of the dossier for this medicinal product.
Beekeeping is an agricultural industry that deals with breeding bees, obtaining honey, wax, and other products. Bees are used for pollination of many types of crops to increase their harvest. Beekeeping products are also used in medicine, pharmacology, chemical, and food industries. One of the essential aspects of successful beekeeping development is ensuring sustainable epizootic well-being from infectious and invasive bee diseases in Ukraine. About 3 million bee colonies are owned by private farms and amateur beekeepers, which give approximately 50-60 thousand tons of commercial honey and 1.2–1.4 thousand tons of wax per year at the current stage of beekeeping development in Ukraine. Honey and bee products from Ukraine received 15 prestigious awards in various categories at the 77th National Honey Show, held in London among many countries in 2008. In recent years, there has been a constant increase in the threat of new little-known invasive and infectious diseases that pose a significant danger to beekeeping worldwide. The invasion of the small hive beetle Aethina tumida belongs to quarantine and hazardous invasive diseases, which have destroyed many bee colonies in several countries worldwide (USA, Canada, African countries, Australia, some European countries – Italy, and Portugal). There are no effective methods of preventing and controlling the small hive beetle. The only way to prevent the invasion of the small hive beetle in Ukraine is constant monitoring and control at the state border of the movement of bee packages and bee products, which may pose a threat to introducing this invasive species into Ukraine.
Based on the analysis of literature sources, monitoring results and our own research, it has been established that viral enteritis occupies a leading place in the infectious pathology of dogs and causes significant damage to animal owners. Viral enteritis, most commonly caused by parvovirus, causes severe gastrointestinal disorders and is one of the most common causes of infectious diarrhea in dogs under 6 months of age. Therefore, given the relevance of this issue, the result of our study is to clarify, supplement and summarize data on the pathomorphological features of the intestinal form of parvovirus infection in dogs. Diagnostic studies to confirm parvovirus enteritis were performed using VetExpert CPV Ag or CPV/CCV Ag rapid tests, as well as by ELISA and PCR. Dissection of dead animals was performed in the conventional sequence. For pathomorphological examination, samples were taken from the gastrointestinal tract, fixed in 10 % neutral formalin solution, followed by dehydration in alcohols of increasing concentration and the procedure of organ compaction according to the generally accepted method. Histological sections 6–10 μm thick were obtained using a microtome. The article presents the results of pathological changes in dogs with parvovirus enteritis, namely macroscopically under the serous membrane of the stomach revealed striped hemorrhages, duodeno-gastric reflux, manifested by gray-green color of the mucous membrane (bile accumulation). Histological methods have shown that the blood vessels of the submucosal base of the mucous membrane are clearly dilated, filled with blood. The mucous membrane is swollen, unevenly colored in all parts of the stomach. In the muscular membrane of the stomach wall, the vast majority of cells were in a state of granular dystrophy, and in some animals, necrotic changes in smooth muscle tissue were recorded. Histochemical studies revealed the development of microscopic changes that indicated hypersecretion of mucus (acidic proteins) in the stomach of sick dogs. No specific macroscopic changes in the colon were recorded. Microscopically, a small amount of cellular detritus with mucus fragments, goblet cell hyperplasia, and a reaction of epithelial cells that were in a state of granular, less often, hydropic dystrophy were recorded on the lumen of the colon on the mucosal surface.
Scientific studies that reflect the evolutionary morphology of the nervous system, namely the spinal nodes of homoeothermic vertebrates, allow us to identify specific patterns, trends, and criteria that relate to the structural organization of the organ at the population and cellular levels of their organization. The evolutionary direction of research into the macro- and microstructure of spinal cord nodes provides an opportunity to investigate the patterns of formation of optimal relationships of their structural components: nerve and glial cells, capillaries concerning the level of development of the organism, and their motor activity. The article contains the results of the morphometric characteristics of the spinal nodes of warm-blooded vertebrates in a comparative aspect: class Aves – Birds (Gallus gallus, forma domestica L., 1758 – domestic chicken); class Mammalia – Mammals (Oryctolagus cuniculus L., 1758 – European krill, Canis lupus familiaris L., 1758 – domestic dog, Sus scrofa, forma domestica L., 1758 – domestic pig, Bos taurus taurus L., 1758 – domestic bull). When performing this work, complex research methods were used: anatomical, neurohistological, morphometric, and statistical. The morphological study of the structural components of the organs of the nervous system was studied on histological sections by the method of light microscopy following the requirements of the international principles of the “European Convention for the Protection of Vertebrate Animals Used in Experiments and for Other Scientific Purposes” (Strasbourg, 1986), a decision of the First National Congress on Bioethics (Kyiv, 2001), Law of Ukraine No. 692 “On the Protection of Animals from Cruel Treatment” (3447-IV) dated February 21, 2006. For histological examination, the newly selected material was immediately fixed in a 10% aqueous neutral formalin solution, with a fixation period of 30 days, followed by step-by-step embedding in paraffin. Using a sled microtome, histological sections with a thickness of 6–10 μm were obtained. The general histological structure and microstructural changes in histological preparations were studied under a light microscope MC (Micros Austria) at magnifications from 70 to 600 times. Photomicrographs of histological preparations were carried out using a CAM V200 video camera mounted in a Micros MC microscope. The work aims to conduct a complex morpho-functional study of spinal nodes in a comparative anatomical series of representatives of higher vertebrates. It was investigated that in phylogenesis, a particular structural and morphofunctional rearrangement of the central and peripheral nervous system organs, namely the spinal nodes, takes place. They differ in shape and size. It was established that the neurocytic organization of the spinal cord nodes of all species of animals studied is characterized by the presence of large, medium, and small nerve cells. Adaptation to various living conditions of animals was formed based on morphometric indicators (the volume of neurocytes and their nuclei), their morphological structures, the density of nerve cells per 0.1 mm2, the number of glial cells per 0.1 mm2, an indicator of nuclear-cytoplasmic ratio, degree of polymorphism chromatophilia. Nissl staining of histopreparations of spinal nodes of homoothermic animals showed that the neuroplasm of nerve cells contains well-defined depths of basophilic substance, compared with lower animals, as evidence of a higher degree of development in nerve cells of the protein-synthesizing apparatus. According to the method of impregnation of spinal cord nodes with silver nitrate in all studied animals, a different intensity of coloring of nerve cells is revealed: dark, light-dark, light, which is related to the peculiarities of species and age neuromorphology, the morphofunctional state of the nervous system and the type of higher nervous activity. The obtained morpho-functional features of the organs of the nervous system are essential not only for evolutionary and comparative morphology but also for developing issues of physiology, pathology, and treatment of peripheral nervous system diseases.
One of the most important directions in the development of modern veterinary medicine is the improvement and implementation of modern methods of diagnosis and prognosis of diseases based on determining the degree of dysfunction and the possibility of correcting the protective functions of the body. for most veterinary clinics, the study of the immunological status of animals is not available, and clinical blood tests make it possible to determine the assessment of the functional state of the immune system by integrated hematological parameters. In the article on the basis of blood formulas that reflect the state of neurohumoral homeostasis and immunological reactivity of the organism, for the first time investigated and established violations of humoral and cellular immune systems, micro- and macrophage systems, reduced nonspecific protection and found relationships between immunity in 23 dogs with leptospirosis, which calculated 19 markers of inflammatory activity, endogenous intoxication, adaptation, nonspecific and immune reactivity. The study was conducted in veterinary clinics in zhytomyr and kyiv, blood for the study was taken from the superficial vein of the forearm v. anterbrachium, medial subcutaneous vein or subcutaneous tibial vein v. saphena. Diagnostic studies to confirm leptospirosis were performed using rapid tests Combo Test IgG Ab/IgM Ab, vetlaboratories and elisa on the basis of a private veterinary laboratory of Llc “Bald” in Kiev. The interpretation of integrated leukocyte blood parameters revealed the development of autoimmune processes with suppression of cellular immunity and the development of endogenous intoxication, which indicates an increase in total (P < 0.01), leukocyte (P < 0.05) and nuclear index (P< 0.001). Suppression of the immune response and increased hypersensitivity of the immediate type based on a decrease in non-specific reactivity by P < 0.001 and inflammation index by P < 0.05. It is proved that integrated hematological parameters of peripheral blood increase the informativeness of the general analysis of blood in dogs with infectious diseases and allow to determine not only the degree of reactivity of the organism, but also to assess the level of endogenous intoxication.
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