The present investigation was conducted for mass multiplication of Jatropha curcas L. through axillary bud culture. For this nodal segment from 3-5 months old nursery grown plants were used as explants for axillary bud culture. The sterilization treatment involving dipping explants in 0.1 per cent HgCl 2 solution for 5 minutes resulted in minimum contamination and maximum establishment of nodal explants. The treatment MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA was the best for culture establishment, shoot proliferation and multiplication of the axillary buds which exhibited highest value in each parameter like establishment (76.1%), number of days taken for shoot initiation (3.1 days), length of longest shoot (6.8 cm), number of leaves on main shoot (7.1) and number of shoots per explant (6.3). Among different treatments for root initiation, half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g AC gave best result in maximum number of rooting percentage (60) with minimum time taken for root initiation (13.3 days), produced maximum number of roots per shoots (5.1) and length of longest root (4.9 cm) when established shoots were treated with it. Such produced plantlets showed nearly cent per cent survival after hardening and acclimatization. It showed that explants surface sterilized with 0.1 per cent HgCl 2 solution for 5 minutes inoculated in MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA and half MS media fortified with 1 mg/L IBA, 3 mg/L NAA and 0.25 g AC were best in shoot establishment and root development respectively for mass multiplication of J. curcas L. through axillary bud culture.
Abstract:The field experiment was conducted at Department of Agronomy, College of Agriculture, OUAT, Bhubaneswar during summer 2014 to find out appropriate hybrids and nutrient management practices for summer sunflower. Application of recommended dose of Fertiliser(RDF) i.e. 60-80-60 kg N, P 2 O 5 -K 2 O ha -1 + ZnSO 4 @ 25 kg ha -1 recorded the maximum capitulum diameter (15.60cm), seed yield (2.17 t ha -1 ), stover yield (4.88 t ha -1 ) and oil yield (0.91 t ha -1 ), while application of RDF + Boron@ 1 kg ha -1 recorded the highest number of total seed (970) and filled seed per capitulum (890) with the lowest unfilled seed (80) and sterility percentage (9.0%). The hybrid 'Super-48' recorded the highest seed and oil yield of 2.17 and 0.91 t ha -1 , respectively, at recommended dose of fertiliser + ZnSO 4 @ 25 kg ha -1 . Experiment was conducted in evaluating the new hybrids in addition to evaluate the response of variety to different nutrient management practices.
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