In vitro mass multiplication of Jatropha (Jatropha curcas L.) through axillary bud culture

Abstract

The present investigation was conducted for mass multiplication of Jatropha curcas L. through axillary bud culture. For this nodal segment from 3-5 months old nursery grown plants were used as explants for axillary bud culture. The sterilization treatment involving dipping explants in 0.1 per cent HgCl 2 solution for 5 minutes resulted in minimum contamination and maximum establishment of nodal explants. The treatment MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA was the best for culture establishment, shoot proliferation and multiplication of the axillary buds which exhibited highest value in each parameter like establishment (76.1%), number of days taken for shoot initiation (3.1 days), length of longest shoot (6.8 cm), number of leaves on main shoot (7.1) and number of shoots per explant (6.3). Among different treatments for root initiation, half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g AC gave best result in maximum number of rooting percentage (60) with minimum time taken for root initiation (13.3 days), produced maximum number of roots per shoots (5.1) and length of longest root (4.9 cm) when established shoots were treated with it. Such produced plantlets showed nearly cent per cent survival after hardening and acclimatization. It showed that explants surface sterilized with 0.1 per cent HgCl 2 solution for 5 minutes inoculated in MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA and half MS media fortified with 1 mg/L IBA, 3 mg/L NAA and 0.25 g AC were best in shoot establishment and root development respectively for mass multiplication of J. curcas L. through axillary bud culture.

Genetic Tracing of Jatropha curcas L. from Its Mesoamerican Origin to the World

“…Second, select elite F 1 or F 2 progenies by phenotypic performance. Finally, use vegetative propagation ( Behera et al, 2014 ) of selected progenies to preserve excellent lines in the heterozygous status. This strategy would be better than crossing among African and Asian Jatropha, or than just transplanting the Mexican one, because of the higher genetic diversity, heterozygosity and climate adaptability.…”

In vitro regeneration protocol through direct organogenesis for Jatropha curcas L. (Euphorbiaceae) accessions in Ethiopia

“…Kozak and Salata (2011) reported that the lower concentration of BAP (0.5 mg/L) produced maximum number of leaves (16.8 leaves) than other concentration of Kinetin, 2iP and TDZ in Rheum rhaponticum. Similarly, Behera et al (2014) obtained highest number of leaf per shoot (7.1) on MS medium supplemented with 1 mg/L BAP and 1 mg/L IAA in Jatropha. More recently Rady et al (2016) also reported that MS supplemented with combination of 0.5 mg/l BA and 0.05 mg/L IBA gave the highest number (14.67) of leaves per proliferated shoot bud of Jatropha after one month of cultivation in the growth room.…”