No abstract

Huinulus lupulus L. (hop) (Cannabidaceae) has been reported to have hypnotic and sedative effects (1-3). But there have been few recent reports concerning the detailed behavioral effects of the hop. Thus, we have investigated the CNS effects of Humulus lupulus (hop) extract using several behavioral tests, such as spontaneous locomotor activity, rotarod test, potentiation of pentobarbital-induced sleep, hypothermic, analgesic, and anticonvulsant tests in mice. Hop extracts (Emil Flachsmann AG) was administered to ICR mice (30-40 g, 6-8 weeks of age) at the doses of 100, 250, or 500 mg/kg i.p., thirty minutes before each test. 1: Spontaneous locomotor activity: Spontaneous locomotor activity (Rhema-Lahoratechnic, Karl KoIb Co.) measured for 1 h was dose-dependently suppressed at doses above 250 mg/kg i.p.. 2: Rotarod performance: The time which mice were able to remain on the rotarod (13 rev. min1, diameter of 5.5 cm, cutoff time of 2 mm) was decreased by 59% and 65%, at the dose of 250 and 500mg/kg i.p., respectively.

Corydalis cava (Fumariacea) is used for the production of preparationes in the theraphy of vegetative distonia and other neurovegetative disturbances [1]. The pharmacological effect of some alkaloids of this plants: bulbocapnine [1], protopine [2,3] and other was investigated or is the subject of many scientists research.The material used for our investigation was from thr lairs of Serbia. Extraction of plant material was carried out by MeOH on 40 C. The alkaloid isolation was performed by means of column chromatography [3]. The course of alkaloid eluation was controlled by thinlayer chromatography on Silica gel G. Seven alkaloids were isolated: bulbocapnine 0.03-0.75 % (dry weight) and domestine, capnoidine, protopine, predisentrine, adlumi-diceine, coptisine. Besides isoboldine and corisamine were found. Our results show that the plant material contained the two fold concentration of copnoidine in relation to the data found in literature.Besides alkaloids fumaric acid was isolated from all plant organs in the quantity 30 % than the ones more isolated from Cosydalis cava from others lairs [5].

From the bulbs, flowers and flowerstalks of Colchicum visiani Parl. of Yugoslavian origin, were isolated and identified the neutral-phenolic and alkaline alkaloids listed in Table II. This investigation showed that the alkaloid content of Colchicum visiani Parl. is similar to that of Colchicum autumnale L., with the exception of a tropolonic alkaloid hR (f)-16 (found as well in Colchicum dörfleri) and a tropolonic alkaloid hR (f)-13, which had so far been unidentified in Colchicum species.