Formalin-fixed paraffin-embedded (FFPE) material presents a readily available resource in the study of various biomarkers. There has been interest in whether the storage period has significant effect on the extracted macromolecules. Thus, in this study, we investigated if the storage period had an effect on the quantity/quality of the extracted nucleic acids and proteins. We systematically examined the quality/quantity of genomic DNA, total RNA, and total protein in the FFPE blocks of malignant tumors of lung, thyroid, and salivary gland that had been stored over several years. We show that there is no significant difference between macromolecules extracted from blocks stored over 11-12 years, 5-7 years, or 1-2 years in comparison to the current year blocks.
Free‐radical copolymerization of methyl acrylate (MA) with N‐p‐chlorophenylmaleimide (NPCMI) and N‐p‐bromophenylmaleimide (NPBMI) initiated by AIBN in cyclohexanone solution at 75°C was studied. The copolymer composition has been determined from the nitrogen content and NMR spectra of the copolymers. The reactivity ratios have been calculated by ML, FR, KT, and JJ methods. Q and e values of NPCMI and NPBMI have also been calculated by the Alfrey–Price Q‐e scheme.
As shown in Figure 5, our present plot is rather similar to that of Baulch et al.15 Lin and Bauer6 obtained k6 = 2.8 X 1012 exp(99.6 kJ) cm6• moL1 2-s_1. In order to explain the negative activation energy, they proposed the following mechanism for the reaction: 0(3P) + CO('Here, C02*(3B2) and C02*('Sg+) represent vibrationally excited C02 in the 3B2 and '2g+ states, respectively. Further, they assumed that the rate-determining step is a transition between the 3B2 state and the * 8+ state and calculated that the crossing point between the 3B2 and * 8+ states is 31.4 kJ/mol below the 3B2 dissociation limit. On the other hand, Clyne and Thrush19 studied the reaction 0(3P) + )(' +) -* C02(%+) + hv (18) Warnatz,
The development of efficient Al batteries is hindered by the major challenge of finding advanced electrode materials, which can deliver higher voltage and storage capacities with ultra-fast charge/discharge rates.
Abstract. The acrylate monomer, 7-acryloyloxy-4-methyl coumarin (AMC) has been synthesized by reacting 7-hydroxy-4-methyl coumarin, with acryloyl chloride in the presence of NaOH at 0-5°C. Copolymers of 7-acryloyloxy-4-methyl coumarin (AMC) with vinyl acetate (VAc) were synthesized in DMF (dimethyl formamide) solution at 70±1°C using 2,2′-azobisisobutyronitrile (AIBN) as an initiator with different monomer-to-monomer ratios in the feed. The copolymers were characterized by Fourier transform infra red (FTIR) spectroscopy. The copolymer composition was evaluated by 1 H-NMR (proton nuclear magnetic resonance) and was further used to determine reactivity ratios. The monomer reactivity ratios for AMC (M1)-VAc (M2) pair were determined by the application of conventional linearization methods such as Fineman-Ross (r1 = 0.6924; r2 = 0.6431), Kelen-Tüdõs (r1 = 0.6776; r2 = 0.6374) and extended Kelen-Tüdõs (r1 = 0.6657; r2 = 0.6256). Thermo gravimetric analysis showed that thermal decomposition of the copolymers occurred in single stage in the temperature range of 263-458°C. The molecular weights of the polymers were determined using gel permeation chromatography.The homo and copolymers were tested for their antimicrobial properties against selected microorganisms.
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