The PH084 gene specifies Pi-transport in Saccharomyces cerevisiae. A DNA fragment bearing the PH084 gene was cloned by its ability to complement constitutive synthesis of repressible acid phosphatase of pho84 mutant cells. Its nucleotide sequence predicted a protein of 596 amino acids with a sequence homologous to that of a superfamily of sugar transporters. Hydropathy analysis suggested that the secondary structure of the PH084 protein consists of two blocks of six transmembrane domains separated by 74 amino acid residues. The cloned PH084 DNA restored the Pi transport activity of pho84 mutant cells. The PH084 transcription was regulated by P1 like those of the PHOS, PHO8, and PHO81 genes. A PH084-lacZ fusion gene produced j}-galactosidase activity under the regulation of Pi, and the activity was suggested to be bound to a membrane fraction. Gene disruption of PH084 was not lethal. By comparison of nucleotide sequences and by tetrad analysis with GAL80 as a standard, the PH084 locus was mapped at a site beside the TUB3 locus on the left arm of chromosome XIII.
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