The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter.

Abstract

The PH084 gene specifies Pi-transport in Saccharomyces cerevisiae. A DNA fragment bearing the PH084 gene was cloned by its ability to complement constitutive synthesis of repressible acid phosphatase of pho84 mutant cells. Its nucleotide sequence predicted a protein of 596 amino acids with a sequence homologous to that of a superfamily of sugar transporters. Hydropathy analysis suggested that the secondary structure of the PH084 protein consists of two blocks of six transmembrane domains separated by 74 amino acid residues. The cloned PH084 DNA restored the Pi transport activity of pho84 mutant cells. The PH084 transcription was regulated by P1 like those of the PHOS, PHO8, and PHO81 genes. A PH084-lacZ fusion gene produced j}-galactosidase activity under the regulation of Pi, and the activity was suggested to be bound to a membrane fraction. Gene disruption of PH084 was not lethal. By comparison of nucleotide sequences and by tetrad analysis with GAL80 as a standard, the PH084 locus was mapped at a site beside the TUB3 locus on the left arm of chromosome XIII.

The expression of GintPT, the phosphate transporter of Rhizophagus irregularis, depends on the symbiotic status and phosphate availability

“…These data raise questions on the dependency of the fungal phosphate transporter (PT) expression on the Pi concentration. To date, high-affinity PT encoding genes that share homology with the yeast high-affinity transporter PHO84 (Bun-Ya et al 1991) have been identified from three AM fungi: Glomus versiforme (GvPT), Rhizophagus irregularis (GintPT), and Glomus mosseae (GmPT) (Harrison and van Buuren 1995;Maldonado-Mendoza et al 2001;Benedetto et al 2005). These genes are expressed in the ERM, indicating a role in Pi acquisition from the soil, as well as in the intraradical mycelium, IRM (Benedetto et al 2005;Balestrini et al 2007;Tisserant et al 2012).…”

A Constitutive Expressed Phosphate Transporter, OsPht1;1, Modulates Phosphate Uptake and Translocation in Phosphate-Replete Rice

“…For the complementation assay, the coding sequence of OsPT1 was amplified by PCR and subcloned into the yeast expression vector p112A1NE to create OsPT1-p112A1NE. The construct was transformed into the yeast Pi uptake-defective mutant MB192 (Bun-Ya et al, 1991). MB192-OsPT1 and control cells were grown to the logarithmic phase and then subjected to the yeast nitrogen base liquid medium containing different Pi concentrations (20, 60, or 100 mM) evenly.…”

Studies of Cytochrome c Oxidase-Driven H+-Coupled Phosphate Transport Catalyzed by the Saccharomyces cerevisiae Pho84 Permease in Coreconstituted Vesicles

“…This high-affinity transporter is encoded by the PHO84 gene, which has been cloned and sequenced (4), shown to be derepressible by phosphate starvation, and regulated at the transcriptional level by the PHO regulatory pathway (5,6). Its synthesis is repressed by P i concentrations of >100 µM (6).…”