The embryology of Swertia minor Knobl. (Gentianinae Gentianaceae) was studied. Features of its development are tetrasporangiate anthers, dicotyledonous type sporangial wall formation, glandular anther tapetum and its dual origin, simultaneous cytokinesis in microsporocytes, tricolporate two-celled pollen, unilocular ovary, forked parietal placentae, tenuinucellar unitegmic anatropous ovules, Polygonum-type megagametogenesis, persistent multiple multinucleate highly enlarged haustorial antipodal cells, nuclear endosperm, Solanad type embryo development, and multiseriate suspensor. Embryological features of taxonomic significance in the Gentianinae are compiled in a review of the pertinent data on the subtribe.

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Over 380 host plant species have been known to develop leaf spots as a result of the fungus Alternaria alternata. It is an aspiring pathogen that affects a variety of hosts and causes rots, blights, and leaf spots on different plant sections. In this investigation, the lipopeptides from the B. subtilis strains T3, T4, T5, and T6 were evaluated for their antifungal activities. In the genomic DNA, iturin, surfactin, and fengycin genes were found recovered from B. subtilis bacterium by PCR amplification. From different B. subtilis strains, antifungal Lipopeptides were extracted, identified by HPLC, and quantified with values for T3 (24 g/ml), T4 (32 g/ml), T5 (28 g/ml), and T6 (18 g/ml). To test the antifungal activity, the isolated lipopeptides from the B. subtilis T3, T4, T5, and T6 strains were applied to Alternaria alternata at a concentration of 10 g/ml. Lipopeptides were found to suppress Alternaria alternata at rates of T3 (75.14%), T4 (75.93%), T5 (80.40%), and T6 (85.88%). The T6 strain outperformed the other three by having the highest antifungal activity against Alternaria alternata (85.88%).

The structure and development of pericarp and seed coat in Rotula aquatica Lour. have been described.The fruit is an ellipsoidal drupe with four pyrenes. The pericarp is divisible into an inner hard and stony endocarp, a pulpy mesocarp and a thin-walled epicarp. The seeds are curved and the seed coat is poorly developed. All the cells of the integument excepting those of the epidermis break down during development. The epidermal cells develop thickenings on their inner tangential and radial walls at later stages. The endocarp forms a hard shell around the seeds and therefore the seeds are not released from the fruit.

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