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Background and Aims: The effect of water stress on berry quality is not fully understood. This study was designed to analyse the differential phenological sensitivity of Tempranillo berry quality to water stress during three phenological stages. Methods and Results: Two-year-old potted Tempranillo vines were exposed to four levels of irrigation (100, 50, 25, and 0% of evapotranspiration) during three phenological stages (Stage I, from anthesis to fruitset; Stage II, pre-veraison; Stage III, post-veraison). Vine water status was monitored by means of leaf water potential measurements. Berry quality was measured at harvest and defined by the following parameters: berry dry weight, soluble solids content, titratable acidity, polyphenol and anthocyanin concentrations in the must. Berry dry-matter accumulation was more sensitive to water stress applied during Stage I and Stage II than in Stage III. Berry quality tended to decrease linearly with increasing water stress during Stage II. During Stage III, berry quality increased linearly for light-to-mild levels of water stress, whereas quality decreased above a certain water-stress threshold (Yleaf = -1.12 MPa). Conclusions: Tempranillo berry quality demonstrated great phenological sensitivity to water stress. Pre-veraison water stress negatively affected berry quality in Tempranillo vines, whereas post-veraison water stress increased quality up to a certain threshold of Yleaf. Significance of the Study: For the first time, this research reports a plant-based water status threshold in Tempranillo vines above which post-veraison water stress can negatively affect berry quality. 268Sensitivity of Tempranillo to water stress

The use of Regulated deficit irrigation (RDI) in almond, applied during the kernel-filling phase, was evaluated over four consecutive years. To determine the reference optimal irrigation rate, three treatments were applied: T-100, which was irrigated by replacing crop evapotranspiration; T-130, which was irrigated by applying 30% more water than in T-100 and T-70, which received 30% less water than T-100. The RDI treatment received the same irrigation rate as T-100, but during the kernelfilling period irrigation was reduced to 20% of T-100. The optimum yield response was observed in treatment T-100, while T-130 trees never improved on T-100 kernel production over the 4 years of the study. During the first two experimental years, kernel dry matter accumulation did not decrease with drought in the RDI treatment. However, both cropping and kernel growth were reduced during the third and fourth years of the experiment. A possible explanation for this decrease could be found in a hypothetical depletion of the carbohydrate reservoir in RDI trees and also to the negative soil water balance that was evident in the T-70 and RDI treatments during winter and spring of the last 2 years. Although yield reductions for RDI trees were significant (20% with respect to T-100), the water savings obtained (about 60% of that applied with respect to T-100), may help to promote the adoption of RDI in areas, where water availability has been reduced. Bearing in mind the water conservation aspect in almond, RDI, as applied in this case, seemed more interesting than a seasonal sustained deficit irrigation strategy like T-70.

To characterize tree responses to water deficits in shallow and deep rooted conditions, parameters developed using daily oscillations from continuously measured soil water content and trunk diameter were compared with traditional discrete monitoring of soil and plant water status in lysimeter and field-grown peach trees [Prunus persica (L.) Batsch `O'Henry']. Evaluation occurred during the imposition of deficit irrigation for 21 days followed by full irrigation for 17 days. The maximum daily available soil water content fluctuations (MXAWCF) taken at any of the four monitored root zone depths responded most rapidly to the deficit irrigation. The depth of the MXAWCF increased with time during the deficit irrigation. Differences relative to a fully irrigated control were greater in the lysimeter than the field-grown trees. Minimum daily trunk diameter (MNTD) and maximum daily trunk shrinkage (MDS) responded sooner than midday stem water potential (stem Ψ), predawn or midday leaf water potential (predawn leaf Ψ and leaf Ψ), or photosynthesis (A). Parameters based on trunk diameter monitoring, including maximum daily trunk diameter (MXTD), correlated well with established physiological parameters of tree water status. Statistical analysis of the differences in the measured parameters relative to fully irrigated trees during the first 10 days of deficit irrigation ranked the sensitivity of the parameters in the lysimeter as MXAWCF > MNTD > MDS > MXTD > stem Ψ = A = predawn leaf Ψ = leaf Ψ. Equivalent analysis with the field-grown trees ranked the sensitivity of the parameters as MXAWCF > MNTD > MDS > stem Ψ = leaf Ψ = MXTD = predawn leaf Ψ > A. Following a return to full irrigation in the lysimeter, MDS and all the discrete measurements except A quickly returned to predeficit irrigation levels. Tree recovery in the field-grown trees was slower and incomplete due to inadequate filling of the root zone. Fruit size was significantly reduced in the lysimeter while being minimally affected in the field-grown trees. Parameters only available from continuous monitoring hold promise for improving the precision of irrigation decision-making over the use of discrete measurements.