In this study, the cd9 gene, a member of the tetraspanin superfamily and involved in various cellular processes, was cloned from Lethenteron camtschaticum. Both real-time PCR and immunohistochemical assays showed broad distribution of cd9 in various L. camtschaticum tissues. In addition, expression levels of Cd9 mRNA were up-regulated in the liver and heart after stimulation by lipopolysaccharide. Flow cytometric analyses demonstrated that cd9 was detected on the leukocytes and that the expression level was higher on granulocytes than on lymphocytes, which implied that cd9 was mainly involved in innate immunity.
M. 2010. Cloning of the gene encoding cucumber lumazine synthase and an analysis of its promoter activity in cucumber. Can. J. Plant Sci. 90: 809Á818. Promoters are an important regulatory element controlling the temporal and spatial expression of genes; thus, they play a critical role in genetic engineering by controlling target gene expression. Cucumber is a widely planted vegetable with a pleasant flavor and high economic value. However, most genetic engineering studies involving cucumber have utilized the CaMV 35S promoter, which mediates ubiquitous target gene expression. To identify a promoter that is highly expressed in cucumber fruit, total proteins from cucumber fruit were analyzed by two-dimensional gel electrophoresis. One spot which is highly expressed in fruit was sequenced from its N-terminus using the Edman degradation method. A total of 10 amino acids (Ala-Val-Arg-His-Ile-Ala-Gly-Ser-Leu-Ala) were sequenced. Based on these 10 residues, a cDNA fragment 905 bp in length was cloned using 3?-and 5?-RACE. The corresponding gene, which encodes 220 amino acids, showed 65Á73% similarity to other plant lumazine synthases without the signal peptide. We also cloned the 2.1-kb upstream promoter sequence of this Cucumis sativus lumazine synthase (CsLS) and analyzed its promoter activity by GUS histochemical and fluorometric assays. Our results indicate that CsLS is highly expressed in cucumber fruit, whereas it is expressed at low levels in cucumber stems and leaves.Key words: Cucumber, promoter, 2-DE, RACE, lumazine synthase Wang, R., Chen, M., Liao, F., Jiang, F., Ma, B., Zhang, Y. et Li, M. 2010. Clonage du ge`ne codant la lumazine synthase et analyse de ses effets sur la croissance du concombre. Can. J. Plant Sci. 90: 809Á818. Les facteurs de croissance jouent un important roˆle de re´gulation, car ils controˆlent l'expression des ge`nes dans le temps et l'espace. Par conse´quent, leur activiteé st de´terminante en ge´nie ge´ne´tique, car ils re´gissent l'expression des ge`nes cible´s. Le concombre est un le´gume abondamment cultive´au gouˆt agre´able et d'une grande valeur e´conomique. Pourtant, la majeure partie des recherches en ge´nie ge´ne´tique sur cette plante recourent au facteur de croissance CaMV 35S, qui influe sur l'expression de nombreux ge`nes. Pour identifier un facteur de croissance s'exprimant vigoureusement chez le concombre, les auteurs ont analyseĺ 'ensemble des prote´ines du le´gume par e´lectrophore`se bidimensionnelle. Une tache s'exprimant vivement chez le fruit a e´teś e´quence´e a`partir du re´sidu amine´terminal selon la me´thode d'Edman. En tout, dix acides amine´s (Ala-Val-Arg-His-IleAla-Gly-Ser-Leu-Ala) ont ainsi e´te´se´quence´s. À partir de ces dix re´sidus, les auteurs ont clone´un fragment d'ADNc de 905 bp aux extre´mite´s 3? et 5? (RACE). Le ge`ne correspondant, qui code 220 acides amine´s, pre´sente une similitude de 65 a7 3 % avec d'autres lumazine synthases ve´ge´tales, en plus de la se´quence-signal. Les auteurs ont e´galement clone´la se´quence du facteur de croiss...
Although the JmjC domain-containing histone demethylases displayed a crucial role in maintaining the homeostasis of histone methylation, while the systematic identification and functional researches of JmjC domain-containing gene family have not been conducted in Mung bean (VrJMJgenes). According to the structural characteristics and phylogenetic relationship with their orthologs from Glycine max, Lotus japonicus, Medicagotruncatula, Arabidopsis thaliana, and Oryza sativa, a total of 18 VrJMJgenes were identified and divided into four clades (KDM3, KDM5. PKDM8, and PKDM9). Interspecies co-collinearity analysis showed the significant JmjC gene duplication events which have occurred during the Papilionoideae evolution. The exon/intron and domain organization of VrJMJgenes from the same clade (or subclade) were similar. All VrJMJ proteins contained a conserved JmjC domain, meanwhile other essential domains also have been found in some specific VrJMJ proteins which responsible for their functions. Numerous abiotic stress and light response related cis-elements associating with transcriptional regulation that were demonstrated in the promoter regions of VrJMJgenes(Pro VrJMJs ). Expression profiles of VrJMJgenes in different tissues showed that most genes displayed a tissue-specific expression in roots or leaves. The acronym RT-qPCR results showed that all VrJMJ genes displayed different degrees of abiotic stress (drought, salinity, and cold) and photoperiodic responses. Furthermore, VrJMJ3 and VrJMJ9 were significantly up-regulated after all three abiotic stress treatments, and VrJMJ13 exhibited a potential function in the photoperiodic regulation of Mung bean flowering. These results provided a clear understanding of VrJMJ genes, and laid a theoretical basis for further verification of their potential biological functions of VrJMJ genes.
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