Cloning of Arctic lamprey <i>Lethenteron camtschaticum cd</i>9 with roles in the immune response

Wu, Fenfang; Su, Peng; Chen, L.; Li, M.; Liu, Xiaomin; Li, Q.

doi:10.1111/j.1095-8649.2012.03299.x

Cited by 14 publications

(6 citation statements)

References 36 publications

(35 reference statements)

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“…Surprisingly, the expression of NICIP in the heart was significantly increased after LPS-stimulated. Our earlier studies reported that CD9 20 and CD29 21 expression level was also significantly higher in lamprey heart after LPS-stimulation. In the interstitial areas of human heart, macrophage-like cells were found and could be stained by CD16, CD32, and CD64 mAbs 22 .…”

Section: Discussionmentioning

confidence: 86%

An inhibitory receptor of VLRB in the agnathan lamprey

Chen

Ren

et al. 2016

Sci Rep

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Lamprey, the primitive jawless vertebrate, uses variable lymphocyte receptor (VLR) as alternative adaptive immune system instead of immunoglobulin (Ig)-based receptors used in jawed vertebrates. In the present study, we characterized a potential inhibitory receptor of VLRB from leucocytes in lamprey. It is a novel ITIM-containing IgSF protein and was therefore named as NICIP. NICIP has two Ig-like domains in extracellular region, a transmembrane domain and two classical ITIM motifs in cytoplasmic domain. It is mainly expressed on the surface of granulocytes and monocytes and can interact with VLRB. In transiently transfected HEK293T cells, it was confirmed again that it could interact with VLRB and the two phosphorylated ITIM motifs could recruit SHP-1 and SHP-2. These results imply that NICIP may play a role as a potential inhibitory receptor of VLRB and involve in negative regulation of immune response mediated by VLRB.

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Section: Discussionmentioning

confidence: 86%

An inhibitory receptor of VLRB in the agnathan lamprey

Chen

Ren

et al. 2016

Sci Rep

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“…In the grass-finished group, 87 were up-regulated while the other 36 decreased their gene activity. Regarding the top 10 most statistically significant genes, CD9 encodes a member of the transmembrane 4 superfamily and associates with lymphoid tissue structure and development, immune cell trafficking, hematopoiesis, lipid metabolism and inflammatory response; studies suggested that CD9 congregating at the T-cell side of the immunological synapse (IS) participates in the immune response and might support the integrin-mediated signaling at the IS [ 34 , 35 ]. SLC4A9, DOCK6 and FGFBP1 mainly interact with cancer.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Profiling of Spleen in Grass-Fed and Grain-Fed Angus Cattle

Carrillo

Ding

et al. 2015

PLoS ONE

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The grass-fed cattle obtain nutrients directly from pastures containing limited assimilable energy but abundant amount of fiber; by contrast, grain-fed steers receive a diet that is comprised mainly of grains and serves as an efficient source of high-digestible energy. Besides energy, these two types of diet differ in a large number of nutritional components. Additionally, animals maintained on rich-energy regimen are more likely to develop metabolic disorders and infectious diseases than pasture raised individuals. Thus, we hypothesize that spleen–a relevant immune organ–may function differently under disparate regimes. The objective of this study was to find the differentially expressed genes in the spleen of grass-fed and grain-fed steers, and furtherly explore the potential involved biopathways. Through RNA sequencing (RNA-Seq), we detected 123 differentially expressed genes. Based on these genes, we performed an Ingenuity Pathway Analysis (IPA) and identified 9 significant molecular networks and 13 enriched biological pathways. Two of the pathways, Nur77 signaling in T lymphocytes and calcium-induced T lymphocyte apoptosis which are immune related, contain a pair of genes HLA-DRA and NR4A1 with dramatically altered expression level. Collectively, our results provided valuable insights into understanding the molecular mechanism of spleen under varied feeding regimens.

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“…The fact that LPS/ConA up-regulates Lj-HMGB2 expression suggests that Lj-HMGB2 triggers the inflammatory response in the lamprey hearts, lymphocyte-like cells and intestines. Our research group had reported several mRNA expression results by real-time PCR, such as CD29 (Wu et al 2010) and CD9 (Wu et al 2012). After the stimulations with LPS/ConA, these mRNA expression levels increased significantly in the heart.…”

Section: Discussionmentioning

confidence: 99%

Cloning and expression analysis of a novel high-mobility group box 2 homologue from Lampetra japonica

Pang

Xiao

Zhao

et al. 2013

Fish Physiol Biochem

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High-mobility group box 2 (HMGB2) is a nonhistone architectural protein that plays important roles in many biological processes. In this study, we cloned a homologue of the HMGB2 from the lymphocyte-like cells of Lampetra japonica (L. japonica). Sequence analysis reveals that L. japonica HMGB2 contains two highly conserved motifs and shares more than 70 % identity with the homologues from other vertebrate species. Subsequently, Lj-HMGB2 was subcloned into the pET-28a(+) and pIRES2 AcGFP1-Nuc vector and expressed in Rosetta blue (DE3) and Hela cell lines, respectively. The recombinant L. japonica HMGB2 (rLj-HMGB2) with apparent molecular mass of 22 kDa was further purified by His-Bind affinity chromatography. Real-time quantitative PCR indicates that the expression level of Lj-HMGB2 was particularly up-regulated in intestines after challenged with lipopolysaccharide, while up-regulated in lymphocyte-like cells and heart after challenged with concanavalin A in vivo. In addition, rLj-HMGB2 could induce the generation of proinflammatory mediators in the activated human acute monocytic leukemia cell line (THP1), which suggested that Lj-HMGB2 may participate in the immune response of the lampreys.

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Cloning of Arctic lamprey Lethenteron camtschaticum cd9 with roles in the immune response

Cited by 14 publications

References 36 publications

An inhibitory receptor of VLRB in the agnathan lamprey

An inhibitory receptor of VLRB in the agnathan lamprey

Transcriptomic Profiling of Spleen in Grass-Fed and Grain-Fed Angus Cattle

Cloning and expression analysis of a novel high-mobility group box 2 homologue from Lampetra japonica

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