The antibacterial mechanism of a Cinnamomum cassia essential oil from Vietnam and of its main component (trans-cinnamaldehyde, 90% (m/m) of C. cassia essential oil) against a Listeria innocua strain was investigated to estimate their potential for food preservation. In the presence of C. cassia essential oil or trans-cinnamaldehyde at their minimal bactericidal concentration (2700 μg·mL(-1)), L. innocua cells fluoresced green after staining with Syto9® and propidium iodide, as observed by epifluorescence microscopy, suggesting that the perturbation of membrane did not cause large pore formation and cell lysis but may have introduced the presence of viable but nonculturable bacteria. Moreover, the fluidity, potential, and intracellular pH of the cytoplasmic membrane were perturbed in the presence of the essential oil or trans-cinnamaldehyde. However, these membrane perturbations were less severe in the presence of trans-cinnamaldehyde than in the presence of multicomponent C. cassia essential oil. This indicates that in addition to trans-cinnamaldehyde, other minor C. cassia essential oil components play a major role in its antibacterial activity against L. innocua cells.
Taking into account interactions between aroma compounds and food components is necessary to better manage the flavoring of food products. These interactions occur at a molecular level and reflect changes, at a macroscopic level, in thermodynamic equilibria, such as solubility or volatility. The rate of transfer of an aroma compound from the liquid to the vapor phase can be affected as well. The behavior of aroma compounds in water and lipid solutions was studied in two complementary ways, a thermodynamic and a kinetic approach (headspace analysis). The transfer rate of volatiles at the liquid-water interface does not only depend on the hydrophobicity of the aroma compounds. Vapor-liquid partition and activity coefficients show the presence of solute-solvent interactions. The Gibbs free energy values indicate their physicochemical nature. JAOCS 75, 127-130 (1998).
In Northern Vietnam, small‐scale cassava starch processing is conducted in densely populated craft villages, where processors face difficulties to expand their activities. Three different processing systems were studied among a cluster of three communes in the Red River Delta, producing up to 430 t of starch (at 55% dry matter) per day. The first system, type A, is a cylindrical rasper and a manual sieve, the second, type B, is a cylindrical rasper and stirring‐filtering machine and the third, type C, used equipment for both the rasping and filtering stages. Moisture, starch, crude fibers and ash content analysis were carried out on samples collected from the A‐B‐C manufacturing processes to establish the mass balance of starch. Production capacity, water consumption, electrical requirements and capital‐labor costs per tonne of starch (12% moisture) were also reported. A‐B‐C manufacturing processes enabled 75% recovery of the starch present in fresh roots. No significant change was observed in the composition of starch. Upgrading from system A to B and subsequently to C resulted in an increase in the extraction capacities (up to 0.9 t of peeled roots per hour), the extraction efficiencies during the extraction stage (up to 93%), and an increase in the water consumption and electrical power (up to 21 m3 and 55 kWh per tonne of starch, respectively). The highest amount of total solids carried in the waste‐water was obtained with type C (up to 17% of the dry weight of fresh roots, compared to 10% and 13% for type A and B, respectively). This may lead to a higher chemical oxygen demand (COD) and biological oxygen demand (BOD) in waste‐water, which can result in more polluted waste‐water than compared with the type A and B technologies. Upgrading the rasping‐extraction technologies also resulted in higher profits and reduction of labor per tonne of starch (up to 18 US$ and 26 man‐hours respectively). The diagnosis proposed in this study can be applied in different contexts to recommend technological options by considering space, energy and capital‐labor availabilities.
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