Effect of a VietnameseCinnamomum cassiaessential oil and its major componenttrans-cinnamaldehyde on the cell viability, membrane integrity, membrane fluidity, and proton motive force ofListeria innocua

Trinh, Nga-Thi-Thanh; Dumas, Émilie; Thanh, M. Le; Degraeve, Pascal; Amara, Chedia Ben; Gharsallaoui, Adem; Oulahal, Nadia

doi:10.1139/cjm-2014-0481

Cited by 52 publications

(40 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resuscitation can also be mediated by different kinds of chemical stimuli, including sodium pyruvate (Lleo et al, 2001; Pinto et al, 2011; Morishige et al, 2013; Pasquaroli et al, 2013), amino acids (Pinto et al, 2011), and Tween 80 (Trinh et al, 2015). It is worth mentioning that researchers have different views on the resuscitation role of pyruvate.…”

Section: Resuscitation Of Vbnc Cellsmentioning

confidence: 99%

Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens

et al. 2017

View full text Add to dashboard Cite

The viable but non-culturable (VBNC) state, a unique state in which a number of bacteria respond to adverse circumstances, was first discovered in 1982. Unfortunately, it has been reported that many foodborne pathogens can be induced to enter the VBNC state by the limiting environmental conditions during food processing and preservation, such as extreme temperatures, drying, irradiation, pulsed electric field, and high pressure stress, as well as the addition of preservatives and disinfectants. After entering the VBNC state, foodborne pathogens will introduce a serious crisis to food safety and public health because they cannot be detected using conventional plate counting techniques. This review provides an overview of the various features of the VBNC state, including the biological characteristics, induction and resuscitation factors, formation and resuscitation mechanisms, detection methods, and relationship to food safety.

show abstract

Section: Resuscitation Of Vbnc Cellsmentioning

confidence: 99%

Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The increase of MIC observed in whole milk containing 3.6% (w/w) milk fat in comparison to TSB 1% (w/w) is similar to the increase of MIC in emulsified TSB 1% (w/w) containing 2.5% (w/w) sunflower oil and 1.5% (w/w) soya lecithin reported by Trinh et al (2013). Since C. cassia EO anti-Listeria activity is mainly due to trans-cinnamaldehyde which represents 90% (w/w) of its components (Trinh et al 2015), physico-chemical characteristics of transcinnamaldehyde such as its log Po/w value (2.12) might explain why its antibacterial activity is far more affected by the presence of milk fat globules than by the presence of proteins like casein micelles and β-lactoglobulin which represent 80% (w/w) and 10% (w/w) of cow's milk proteins, respectively. Compounds with the highest log Po/w values are likely more susceptible to bind to hydrophobic particles like milk fat globules.…”

Section: Resultsmentioning

confidence: 99%

“…Skimmed, semi-skimmed and whole milk powder were purchased from Régilait (St Martin Belleroche, France). Their respective compositions once resuspended in distilled water are stated in (Trinh et al 2015).…”

Section: Methodsmentioning

confidence: 99%

“…essential oils) antimicrobial molecules can be distinguished. In previous studies, Gaillac red wine powder (BouarabChibane et al 2017) and Cinnamon cassia essential oil (Trinh et al 2015) were selected based on their in vitro antibacterial activity against several pathogenic or food-spoiling bacterial strains. Gaillac red wine powder is rich in phenolics, while trans-cinnamaldehyde is the main component of C. cassia essential oil (EO).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of interaction with food constituents on plant extracts antibacterial activity

Bouarab-Chibane¹,

Oulahal²,

Dumas³

et al. 2018

FSAB

Self Cite

View full text Add to dashboard Cite

Gaillac red wine powder and Cinnamon cassia essential oil were selected for their in vitro antibacterial activity against Staphylococcus aureus CNRZ3 and Listeria innocua LRGIA 01, respectively. In order to assess the potential application of Gaillac wine powder to the preservation of raw meat, its antibacterial activity was assayed in Mueller Hinton broth supplemented with up to 20% (w/w) bovine meat proteins (bovine meat protein content): Gaillac wine powder as well as resveratrol, a stilbene polyphenol present in red wine, lost their antibacterial activity, likely as a result of interactions of Gaillac wine antibacterial molecules with bovine meat proteins at the expense of their interactions with S. aureus CNRZ3 cells. Cinnamon cassia essential oil antibacterial activity assays in Tryptone Soya broth, skimmed, semi-skimmed, and whole milk showed that its antibacterial activity was significantly reduced by milk fat globules but not by milk proteins: it could thus be used for the preservation of skimmed milk. The developed methodology based on the use of microbiological media mimicking the composition of perishable foods or of liquid foods such as sterilized milk with various milk fat contents could be used for the rapid screening of antibacterial plant extracts of interest for perishable foods preservation.

show abstract

“…In this physiological state, VBNC bacteria cannot be cultured on routine media that normally support their growth and proliferation but maintain their cell membrane integrity and reduced metabolic activities, including synthesis of ATP and expression of gene, RNA and transcript (Trinh et al . ). The exact mechanisms underlying the shift of micro‐organisms into a VBNC state are still not fully understood.…”

Section: Introductionmentioning

confidence: 97%

Detection of viable but nonculturable Vibrio parahaemolyticus induced by prolonged cold‐starvation using propidium monoazide real‐time polymerase chain reaction

Yoon

Moon

Choi

et al. 2019

Lett Appl Microbiol

View full text Add to dashboard Cite

Viable but nonculturable (VBNC) Vibrio parahaemolyticus cannot be detected by the standard cultivation‐based methods. In this study, commonly used viability assessment methods were evaluated for the detection of V. parahaemolyticus in a VBNC state. Vibrio parahaemolyticus cells exposed to nutrient deficiency at cold temperature were used for epifluorescence microscopy with SYTO9 and propidium iodide (PI) staining and real‐time polymerase chain reaction (qPCR) with propidium monoazide (PMA), and its resuscitative ability was determined by a temperature upshift in freshly prepared artificial sea water (ASW; pH 7) fluids. Viable cells with intact membranes always exceeded 5·0 log CFU per ml in ASW microcosms at 4°C. After 80 days, cycle thresholds for V. parahaemolyticus ATCC 27969 were 16·15–16·69. During cold‐starvation, PMA qPCR selectively excluded DNAs from heat‐killed cells. However, there may be some penetration of PMA into undamaged cells that persisted in ASW for 150 days, as evidenced by their ability to resuscitate from a VBNC state after a temperature upshift (25°C); V. parahaemolyticus ATCC 33844 and V. parahaemolyticus ATCC 27969 were successfully reactivated from a VBNC state in ASW microcosms containing <5% NaCl, following enrichment in ASW medium (pH 7). Significance and Impact of the Study Few studies have evaluated the characteristics of and detection methods for viable but nonculturable (VBNC) Vibrio parahaemolyticus induced by cold‐starvation. Currently, VBNC cells are routinely detected by SYTO9 and propidium iodide double staining. However, viable cell counts might be overestimated by this approach, suggesting that the fluorescence dyes may be ineffective for accurately determining the viability of bacterial cells. We demonstrated that quantitative real‐time polymerase chain reaction with propidium monoazide, which selectively permeates damaged cell membranes, can be used to obtain viable cell counts of V. parahaemolyticus after its evolution to a VBNC state under cold‐starvation conditions.

show abstract

Effect of a VietnameseCinnamomum cassiaessential oil and its major componenttrans-cinnamaldehyde on the cell viability, membrane integrity, membrane fluidity, and proton motive force ofListeria innocua

Cited by 52 publications

References 35 publications

Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens

Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens

Effect of interaction with food constituents on plant extracts antibacterial activity

Detection of viable but nonculturable Vibrio parahaemolyticus induced by prolonged cold‐starvation using propidium monoazide real‐time polymerase chain reaction

Contact Info

Product

Resources

About