Poly(vinylidene fluoride-trifluoroethylene, P(VDF-TrFE)) and P(VDF-TrFE)/barium titanate nanoparticle (BTNP) films are prepared and tested as substrates for neuronal stimulation through direct piezoelectric effect. Films are characterized in terms of surface, mechanical, and piezoelectric features before in vitro testing on SH-SY5Y cells. In particular, BTNPs significantly improve piezoelectric properties of the films (4.5-fold increased d31 ). Both kinds of films support good SH-SY5Y viability and differentiation. Ultrasound (US) stimulation is proven to elicit Ca(2+) transients and to enhance differentiation in cells grown on the piezoelectric substrates. For the first time in the literature, this study demonstrates the suitability of polymer/ceramic composite films and US for neuronal stimulation through direct piezoelectric effect.

In this letter, we report on the fabrication, the characterization, and the in vitro testing of structures suitable for cell culturing, prepared through two-photon polymerization of a nanocomposite resist. More in details, commercially available Ormocomp has been doped with piezoelectric barium titanate nanoparticles, and bioinspired 3D structures resembling trabeculae of sponge bone have been fabricated. After an extensive characterization, preliminary in vitro testing demonstrated that both the topographical and the piezoelectric cues of these scaffolds are able to enhance the differentiation process of human SaOS-2 cells.

The influence of interfacial interactions and annealing time on dynamics of the α-relaxation in ultrathin poly(vinyl acetate) films deposited on different substrates has been studied using local dielectric spectroscopy at ambient pressure and controlled humidity. After annealing at 323 K for about 3 days, for polymer films supported on gold and aluminum substrates, an increase of the relaxation rate with decreasing film thickness below 30−35 nm was observed, whereas for films deposited on silicon substrates a thickness-independent dynamics was found for films as thin as 12 nm. The difference in size effect on dynamics of the films could reasonably be related to the difference in interfacial energy between polymer films and substrates, even though a criterion simply based on interfacial energy cannot be used to explain all the results. In fact, further annealing at a higher temperature evidenced an annealing-dependent dynamics in films prepared on aluminum substrates consistent with the presence of long-living metastable states at the polymer/substrate interface. The lifetime of such metastable states seems related to the nature of the substrate as well as to the molecular weight of the polymer.

The effect of severe confinement on the dynamics of three different generations of hyperbranched polyesters of the Boltorn family is investigated by dielectric relaxation spectroscopy (DRS). The polymer chains are intercalated within the galleries of natural montmorillonite (Na+-MMT), thus forming 1 nm polymer films confined between solid walls. The structure of the nanocomposites is studied with X-ray diffraction and the thermal behavior of the polymers in bulk and under confinement is determined by differential scanning calorimetry. The glass transition temperatures of the polymers show a clear dependence on the generation whereas the transition is completely suppressed when all the polymer chains are intercalated. The dynamic investigation of the bulk polymers reveals two sub-Tg processes, with similar behavior for the three polymers with the segmental relaxation observed above the Tg of each polymer. For the nanocomposites, where all the polymer chains are severely confined, the dynamics show significant differences compared to that of the bulk polymers. The sub-Tg processes are similar for the three generations but significantly faster and with weaker temperature dependence than those in the bulk. The segmental process appears at temperatures below the bulk polymer Tg, it exhibits an Arrhenius temperature dependence and shows differences for the three generations. A slow process that appears at higher temperatures is due to interfacial polarization.