The purpose of this study has been to determine the effect of storage on grain quality of three oat cultivars selected in Serbia-Dunav, Vrbas and NS Tara. Freshly harvested oats were stored at the temperature of 25±2 °C for 12 and 20 months. There was a significant decrease in the volume mass (458.4 to 408.9 kg/m-3) and the absolute mass of grains (26.6 to 24.2%) during the storage. The germination potential of the grains decreased over time (96.2-94.1%). The changes in the acid degree values (3.77-3.99 ml 1(Na OH/100 g) are highly significant (p<0.01). Initially, the pH level of the fresh samples was 6.2, and it decreased to 5.8 after 20 months. Genotypes and volume mass have great effect on storage duration (η 2 =0.8130 η 2 =0.7621 and η 2 =0.6780). The interaction between the studied factors did not show statistically significant effects on the change in germination of oat grains (p>0.05). What mostly affects an increase in the acid degree value of oat grains is storage duration, followed by a genotype and the interaction between these two factors. The studied oat genotypes show no significant differences in glassiness (p>0.05).
The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36ºC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation.
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