GENETICS: EDWARDSON AND CORBETT PROC. N. A. S.phan, but higher concentrations are needed than is the case with the other accumulated products. Its appearance coincides with the loss or repression of tryptophan synthetase activity in the culture. The relationship of this compound to the tryptophan pathway has not yet been ascertained. It does not interfere with scoring or the identification of strains. 21 Procedures for the assay of tryptophan systetase in whole cells were adapted from those of R. Eisenstein and C. Yanofsky, unpublished results.22 Spizizen, J., unpublished data. 23 In these experiments the efficiency of transformation was very low, about l/loon that using strain T3, yet the per cent of recombinants was the same.Failure to transmit cytoplasmic male sterility in plants by inoculation or by grafting has been reported in corn by Rhoades,I in tobacco by Sand,2 and in petunia by Everett.3 Frankel,4 on the other hand, grafted cytoplasmic male sterile and fertile petunia plants and reported that "grafting induced changes in the fertile scion that resulted in the appearance of cytoplasmic sterility in its progeny."Frankel's report was cited by Gloustshenko5 as an example of graft hybridization.

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