Undulant fever as a public health problem is unique in many ways. It has been recognized as such only recently. That the principal source of the disease is in domestic stock no well informed observer doubts. There is no uniformity of opinion concerning the relative importance of channels through which the infection may reach man from animals. No student of the subject denies that infected milk may result in spreading undulant fever among humans. A recent analysis of 155 cases of sickness that occurred during 1929 and 1930 in Illinois and which were clinically and serologically diagnosed as undulant fever cast a very strong suspicion on raw milk supplies as the agent of transmission in a significant percentage of the total incidence. Observers elsewhere have found evidence that infected milk may be an important means of transmitting the disease. Furthermore, undulant fever prevalence may be on the up curve, potentially at least. If nothing is done to control the disease a great endemic wave of this ailment among men in the not far distant future is a catastrophe which is well within the realm of the possible. On the other hand a relatively small amount of judicious energy spent now in research and control may offset that possibility. For these reasons it seems of the greatest importance to bring to light all possible knowledge about the cause of undulant fever and means of controlling the spread of it. The accompanying report is a contribution to an important phase of this knowledge. Some controversy about the efficacy of pasteurization in destroying the causative organisms of undulant fever has arisen. Doubts created by this controversy will survive until the matter is settled by indisputable scientific experimentation. This report might be accepted as closing the chapter on one phase of the necessary experimentation.-ANDY HALL, Director of Public Health, Chairman, State Undulant Fever Committee.] Results of investigations made in recent years show that (a) cattle may be spontaneously infected with Brucella strains of Assistant Bacteriologist, State Department of Agriculture.
development in Canada field pea when the plant is grown in water or soil cultures; (3) A study of the influence of various environmental conditions on the infecting power of the organism. METHOD OF INVESTIGATION Organism Bacillus radicicola from Canada field pea was used for all the experiments except number 11, for which the organism from alfalfa was used. The # Number of Nodules per Plant in Second Test, Arranged in Numerical Order Medium Organisms living or not living* Medium 334 + 1 per cent Witte's peptone.. .. Medium 334 + 5 per cent gelatin Medium 334 + 0.2 per cent calcium nitrate.. Medium 334 + 2 per cent glycerin Medium 334 + 0.5 per cent amygdalin Medium 334 + 0.6 per cent potassium nitrate. Medium 334 + 10 per cent cane sugar Cornstarch Wheat middlings Medium 334 + 2 per cent galactose Medium 334 + 40 per cent cane sugar Medium 334 + 0.1 per cent asparagin Medium 334 + 2 per cent dextrose Medium 334 + 2 per cent cane sugar Wheat bran Sandy soil Compost Fresh cow feces Medium 334 + 2 per cent cane sugar Partly decomposed cow feces Muck Canada field pea roots Medium 334 + 0.1 per cent potassium nitrate. Medium 334 + 0.1 per cent calcium nitrate.. . Medium 334+1 per cent Merck's peptone.. . Medium 334 + 0.2 per cent potassium nitrate. M<' ium 331 + 0.6 per cent calcium nitrate.. Medium 334 + 0.2 per cent Merck's peptone. Medium 334 + 20 per cent cane sugar Medium 331+1 per cent salicin Medium 334 + 0.5 per cent resorcin Canada field pea hay Medium 334 + 0.2 per cent phloroglucin Medium + 2 per cent lactone Sawdust Medium + 2 per cent Witte's peptone.. . .
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