Physiological studies of Bacillus radicicola of Canada field pea

Abstract

development in Canada field pea when the plant is grown in water or soil cultures; (3) A study of the influence of various environmental conditions on the infecting power of the organism. METHOD OF INVESTIGATION Organism Bacillus radicicola from Canada field pea was used for all the experiments except number 11, for which the organism from alfalfa was used. The # Number of Nodules per Plant in Second Test, Arranged in Numerical Order Medium Organisms living or not living* Medium 334 + 1 per cent Witte's peptone.. .. Medium 334 + 5 per cent gelatin Medium 334 + 0.2 per cent calcium nitrate.. Medium 334 + 2 per cent glycerin Medium 334 + 0.5 per cent amygdalin Medium 334 + 0.6 per cent potassium nitrate. Medium 334 + 10 per cent cane sugar Cornstarch Wheat middlings Medium 334 + 2 per cent galactose Medium 334 + 40 per cent cane sugar Medium 334 + 0.1 per cent asparagin Medium 334 + 2 per cent dextrose Medium 334 + 2 per cent cane sugar Wheat bran Sandy soil Compost Fresh cow feces Medium 334 + 2 per cent cane sugar Partly decomposed cow feces Muck Canada field pea roots Medium 334 + 0.1 per cent potassium nitrate. Medium 334 + 0.1 per cent calcium nitrate.. . Medium 334+1 per cent Merck's peptone.. . Medium 334 + 0.2 per cent potassium nitrate. M<' ium 331 + 0.6 per cent calcium nitrate.. Medium 334 + 0.2 per cent Merck's peptone. Medium 334 + 20 per cent cane sugar Medium 331+1 per cent salicin Medium 334 + 0.5 per cent resorcin Canada field pea hay Medium 334 + 0.2 per cent phloroglucin Medium + 2 per cent lactone Sawdust Medium + 2 per cent Witte's peptone.. . .

The Effect of Soluble Nitrogenous Salts on Nodule Formation1

“…According to Prucha ( 14) small amounts of potassium nitrate, calcium nitrate, ferric nitrate, ferric chloride, ammonium chloride, potassium chloride, and peptone inhibit nodule development, while the addition of tannic acid, magnesium sulfate, monopotassium phosphate, potassium hydroxide, dicalcium phosphate, and calcium sulfate show a beneficial effect.…”

Influence of Various Nitrogenous Compounds and Mannitol on Nodule Formation by Clover

“…RITTER (14) reported that in soil, sucrose had no effect on the formation of nodules by lupinies. PRUCHA (13), however, found that more than 2 gin. of sucrose added to 300 gm.…”

Notes on the Flagellation of the Nodule Bacteria of Leguminosae

“…Fred (1918) has found on the alfalfa organism several peritrichic flagella and on the lupine organism one or rarely two. Prucha (1915) has found several flagella on the organism from the Canada field pea.…”