No abstract

No abstract

No abstract

No abstract

No abstract

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. BTV-infected farms with an average seroprevalence of 57%. In addition, bulk milk 31 samples of the same farms, and historically BT-negative samples were tested. 32Compared to the ELISA for sera, the relative specificity and sensitivity of the ELISA 33 for milk samples is 96.5% and 98.9%, respectively when using a S/P% cut-off value 34 of 50% as advised by the manufacturer. The optimal cut-off value was found at S/P% 35 of 90% revealing an optimal specificity (99.0%) combined with an optimal sensitivity 36 (98.1%). Titres in positive individual milk samples ranged from 1 to 2048 with a peak 37 titre of 128. Bulk milk samples contained antibodies with titres ranging from 64 to 38 39The ELISA for milk samples was found to be a reliable and robust test. This 40 diagnostic tool is very useful, and may replace the ELISA for serum samples as first 41 choice in order to get insight into the status of lactating individual animals and 42 therewith of the entire herd with respect to BTV infection. 43 44 45

No abstract

No abstract