Summary:
The effect of degree of water stress in Avena sativa on diclofop‐methyl efficacy was assessed. Within 24 h of applying stress by adding mannitol to the root medium (0 to 12.5%), the rate of leaf extension of the youngest leaves (leaves 3 and 4) decreased with increasing mannitol concentration. Without water stress, application of diclofop‐methyl had little effect on extension rate of leaves 3 and 4 during the first 4 days after spraying. Subsequently, it caused a significant decrease in the extension rate of leaf 4 with more pronounced effects on later leaves. Diclofop‐methyl had little effect on leaf extension rate of plants given mannitol. Shoot dry weight at harvest for unsprayed plants decreased with increased mannitol concentration and for sprayed plants it was greater without mannitol than with mannitol (all levels). However, at mannitol concentrations greater than 4%, shoot dry weight for sprayed and unsprayed plants was not significantly different. Sprayed plants exposed to 2–8.5% mannitol produced seed heads but those at zero mannitol did not. When the root medium of all treatments was flushed of mannitol one week after spraying, then main‐tained without mannitol, shoot dry weight at harvest for unsprayed plants decreased slightly with increased mannitol concentration applied initially. However, shoot dry weight for sprayed plants increased with increased mannitol concentration. Without mannitol two weeks after spraying, chlorophyll concentrations of leaves 3 and 4 were greater and water saturation deficit (WSD) values were lower for unsprayed plants than sprayed plants but there were no differences at 6.2% mannitol. It is proposed that tolerance to diclofop‐methyl by A. sativa as a result of water stress, is primarily due to a decreased rate of leaf expansion resulting in lower demand for membrane synthesis and less strain on membranes damaged by the herbicide.
Avena sativa cv. Amuri fed either low or high nitrate was sprayed with diclofopmethyl ( 1 kg a.i. ha-I) at the three leaf stage. The short term effects of the herbicide on chlorophyll concentration of leaves (laminae) and short and long term effects on d.wt of the component plant parts were determined by comparison with unsprayed plants.For unsprayed and sprayed plants, total leaf d.wt approximately doubled during the first twelve days after commencing treatments. Growth was substantially greater at high nitrate than low nitrate. For unsprayed plants, the increase in total leaf d.wt was due primarily to growth of leaf 3 but for sprayed plants it was due to growth of leaves 1 and 2. Twelve days after commencing treatments, d.wt of leaves 1 and 2 was substantially greater for sprayed plants than for unsprayed plants given similar nitrate, while chlorophyll concentration was substantially less. Leaf 3 d.wt and chlorophyll concentration were substantially greater in unsprayed plants than in sprayed plants given similar nitrate. For unsprayed plants, values were greater at high nitrate than low nitrate, for sprayed plants the converse was the case.Forty nine days after commencing treatments, unsprayed plants had a greater total plant d.wt than sprayed plants given similar nitrate. Total plant d.wt for unsprayed plants was greater at high nitrate than low nitrate, the opposite was the case for sprayed plants. Unsprayed plants a t both nitrate levels and sprayed plants given low nitrate produced seed heads but sprayed plants given high nitrate did not. Diclofop-methyl at a rate of 0.3 kg a.i. ha-' stopped seed head production at high nitrate. Retention and uptake of diclofop-methyl were not significantly different at low and high nitrate. At 1 kg a.i. ha-l diclofop-methyl, plants switched from low to high nitrate at spraying showed damage similar to that shown by plants given high nitrate throughout. Addition of 200 vg GA into the leaf sheaths two days prior to spraying increased the efficacy of diclofop-methyl at low nitrate.It is proposed that increased efficiency of diclofop-methyl at high nitrate is due to increased leaf damage caused by a greater rate of leaf expansion.
SUMMARYThe experimental growth retardant WL83801, applied as a root drench, had a rapid and persistent effect in retarding the growth and development of leaves in L. perenne. Leaves of the main shoot were greatly reduced in length, were broader, and appeared faster than in control plants. The rate of extension of individual leaves was greatly reduced in retarded plants but still followed a diurnal pattern that closely corresponded with temperature. There was evidence that leaf extension was far less responsive to temperature in treated plants. At the cellular level WL83801 had no significant effect on leaf blade cell number, thus reductions in leaf length were associated with the retardation of cell elongation. Changes in leaf structure were also observed.These changes in the pattern of leaf growth and development are discussed in relation to the primary mode of action of the growth retardant in interfering with gibberellin biosynthesis.
A specially-designed, pre-moistened multilayered breathable fabric failed to promote evaporative cooling compared to negative control. Cold water baths may need to be repeated throughout recovery to optimise their effect.
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