Infection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) has been confirmed in several studies by serological and molecular techniques. In order to determine the presence of persistently infected animals and circulating species and subtypes of BVDV we conducted this study on a buffalo herd, whose habitat was shared with bovine cattle (Bossp.). Our serological results showed a high level of positivity for BVDV-1 and BVDV-2 within the buffalo herd. The molecular analyses of blood samples in serologically negative animals revealed the presence of viral nucleic acid, confirming the existence of persistent infection in the buffaloes. Cloning and sequencing of the 5' UTR of some of these samples revealed the presence of naturally mix-infected buffaloes with at least two different subtypes (1a and 1b), and also with both BVDV species (BVDV-1 and BVDV-2).
<p>Con el objetivo de efectuar los primeros diagnóstico serológicos para determinar los serovares prevalentes de Leptospira sp en búfalos, se realizó un muestreo en animales pertenecientes a siete rodeos bubalinos de la región noroeste de la Provincia de Corrientes, Argentina (n=550). Los sueros sanguíneos fueron procesados por microaglutinación con antígenos vivos mantenidos en cultivos especiales (MAT). Se identificaron los serovares tarassovi y wolfii.</p>
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