The yield coefficient Yx/s was correlated with variables such as temperature (22.8-29.2 oC), concentrations of corn steep liquor (6.4-57.4 g/L), ammonium sulfate (5.6-18.4 g/L), sucrose (4.0-36.0 g/L) and soybean oil (3.0-7.0 g/L), all important variables in the growth stage of the Penicillium chrysogenum fungus. An empirical model was obtained by response surface statistical experimental design. The tests were performed with a complex culture medium in a rotatory shaker. The highest Yx/s values were obtained at low sucrose concentrations (~4.0 g/L) and high corn steep liquor concentrations (~57.4 g/L). Temperature, ammonium sulfate and soybean oil concentrations showed no significant effect on Yx/
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