“…Optimization studies are carried out using response surface methodology (RSM) which is a mathematical and statistical technique widely used to determine the effects of several variables and their interactions and to optimize different biotechnological processes (Oprime and Suazo, 1997;Burkert et al, 2006;Zafar et al, 2010;Coelho et al, 2011;). RSM has been extensively applied to optimize culture medium and other process parameters for the production of β-fructofuranosidase (Chen 1998), lipase (He and Tan 2006;Liu et al, 2006), tannase (Battestin and Macedo 2007), α-amylase (Rao and Satyanarayana 2007), α-cyclodextrin glucanotransferase (Ibrahim et al, 2005), dextran dextrinase (Naessens et al, 2004) and chitinase (Nawani and Kapadnis 2005).…”