This study showed that a high accuracy of IHC could be obtained for the determination of HER2 status in all laboratories using their in-house IHC technique, provided that a calibration process was performed. Antigen retrieval procedure, high dilutions of anti-HER2 antibody and the use of specific controls were crucial for HER2-IHC calibration. A 95% accuracy rate of IHC, using FISH as gold standard, was obtained by considering immunolabelling HER2-IHC results as a continuous variable, and taking 60% invasive stained cells as the cut-off for HER2 over-expression.
After total hip arthroplasty (THA), many studies report that a small percentage of patients mention painful symptoms, whose origin remains more or less obscure. We investigated 1,000 patients who had undergone a THA at least one year before their inclusion in the survey protocol. Among these 1,000 patients, 64 were complaining of pain in the region of the operated hip. These were later examined and investigated, both clinically (physical and psychiatric examination) and paraclinically (radiography, biology). Those requiring it received adequate treatment and the others were only regularly followed up. We identified the cause of all but one patient's pain. In all cases except one, the symptoms of pain without physical cause had a psychiatric origin. The results of our study show that, if the clinical picture is not perfectly clear, a psychiatric screening before surgery could contribute to decreasing the incidence of pain syndrome after THA.Résumé Comme le rapportent de nombreuses études, un pourcentage limité de patients conservent des douleurs après prothèse totale de hanche, douleur dont l'origine reste relativement obscure. Nous avons analysé 1000 patients ayant bénéficié d'une prothèse totale de hanche dont le recul était au moins égal à un an. Parmi ces 1000 patients, 64 se plaignaient de douleurs dans la région de la hanche. Ces patients ont été explorés sur le plan clinique, paraclinique et psychiatrique. Certains ont bénéficié d'un traitement, d'autres ont été seulement régulièrement suivis. Nous avons identifié l'origine de la douleur chez tous les patients (sauf un). Les douleurs qui n'avaient pas une origine somatique étaient d'origine psychiatrique. Les résultats de notre étude montrent que si le tableau clinique n'est pas tout à fait clair, un examen psychiatrique réalisé avant la chirurgie peut contribuer à diminuer les syndromes douloureux après intervention chirurgicale.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.