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In this work we compare previously measured energy barriers over the course of temperature with the results of simulations of the behaviour of the energy barriers. For the measurements the temperature dependent magnetorelaxation method (TMRX) was used. For the simulations of the energy barrier distribution we have used the real particles properties such as anisotropy and core size volume of the fractions of two magnetically fractionated ferrofluids. There is a good agreement between the simulated behaviour and the experimental obtained results. The influence of the particle volume concentration and agglomeration on the energy barrier distribution has been investigated. Finally the simulations confirm a previously published explanation for an experimentally obtained relaxation effect.