We regret to report the death of M. Sc. Maria Borkowska after the completion of this work.
AbstractThe lnesophyll protoplasts were isolated from the Solanum tuberosum (S. tbr) clones of different ploidy level (4x Bzura cv., 2x H-8105, and 2x ZEL-1. 136) as well as from the wild species: S. bulbocastamlm (S. blb, 2x) and two accessions of S. ~ligrum (S. ngr, 6x). Additionally, the protoplasts were isolated from the cell suspensions of Bzura cv. and H-8105 clone. The conditions of protoplast isolation as well as the media for their culturing and regeneration, were selected and optimized for the studied gcnotypes. For mesophyll protoplasts, the shooting calli were produced by all the cultured protoclones except that of S. bulbocastamm~. The shoots excised from the protoplastderived calli developed into whole plants in all the studied potato clones but only in one accession of S. nigrum, i.e.S, ngr var. gigar~tea. As for suspension-cell-derived protoplasts, only H-8 i05 clone produced the regenerative type of calli, though normal shoots could not be obtained. The regenerative capacity of the protoplasts isolated from leaves and cell suspensions is compared and discussed. G. 1987 Variations ol"morphogenetic behavior and plant regeneration in cultured protoplasts of Solanum nigrum. Plant Sci., 52:117-126.
Sihachakr D., Ducreux
Soybean β-1,3-endoglucanase represents a model system for studies on early plant responses to infection by fungal pathogens, and it has been implicated in the release of elicitors from fungal cell walls. In the present study, potato plants were transformed with the soybean β-1,3-endoglucanase cDNA via Agrobacterium delivery system. The transfer of the gene into potato genome was confirmed by (i) PCR amplification, (ii) Northern blot analyses, and (Hi) an increase in the activity of β-1,3-endoglucanase in transgenic plants. The transformation resulted in an increased resistance of selected transgenic plants to infection by Phytophthora infestans, an important pathogen.
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