Transgenic Potato Plants Expressing Soybean ß-1,3-Endoglucanase Gene Exhibit an Increased Resistance to Phytophthora infestans

Borkowska, M.; Krzymowska, Magdalena; Talarczyk, Andrzej; Awan, M. F. M.; Yakovleva, L. A.; Kleczkowski, K; Wielgat, Bernard

doi:10.1515/znc-1998-11-1212

Cited by 24 publications

(4 citation statements)

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“…Protection against different filamentous pathogens in transgenic plants due to basic β-1,3-glucanase overexpression, has been observed for R . solani 57 , Phytophthora infestans 58 , Fusarium sp . 59 , Cercospora arachidicola and Aspergillus flavus 60 .…”

Section: Discussionmentioning

confidence: 99%

Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials

Boccardo

Segretin

Hernández

et al. 2019

Sci Rep

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Plants are continuously challenged by pathogens, affecting most staple crops compromising food security. They have evolved different mechanisms to counterattack pathogen infection, including the accumulation of pathogenesis-related (PR) proteins. These proteins have been implicated in active defense, and their overexpression has led to enhanced resistance in nuclear transgenic plants, although in many cases constitutive expression resulted in lesion-mimic phenotypes. We decided to evaluate plastid transformation as an alternative to overcome limitations observed for nuclear transgenic technologies. The advantages include the possibilities to express polycistronic RNAs, to obtain higher protein expression levels, and the impeded gene flow due to the maternal inheritance of the plastome. We transformed Nicotiana tabacum plastids to co-express the tobacco PR proteins AP24 and β-1,3-glucanase. Transplastomic tobacco lines were characterized and subsequently challenged with Rhizoctonia solani , Peronospora hyoscyami f.sp. tabacina and Phytophthora nicotianae . Results showed that transplastomic plants expressing AP24 and β-1,3-glucanase are resistant to R . solani in greenhouse conditions and, furthermore, they are protected against P . hyoscyami f.sp. tabacina and P . nicotianae in field conditions under high inoculum pressure. Our results suggest that plastid co- expression of PR proteins AP24 and β-1,3-glucanase resulted in enhanced resistance against filamentous pathogens.

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Section: Discussionmentioning

confidence: 99%

Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials

Boccardo

Segretin

Hernández

et al. 2019

Sci Rep

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“…Increased resistance against Phytophthora infestans due to increase in glucanase activity in transgenic potato plants expressing soybean glucanase gene have been reported (Borkowoska et al 1998). Reduction in the disease index values was observed in transgenic tobacco plants expressing barley glucanase that correlated well with the levels of glucanase accumulation (Jach et al 1995).…”

Section: Introductionmentioning

confidence: 90%

Increased resistance to fungal wilts in transgenic eggplant expressing alfalfa glucanase gene

Singh

Ambroise

Haïcour

et al. 2014

Physiol Mol Biol Plants

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The wilt diseases caused by Verticillium dahliae and Fusarium oxysporum are the major diseases of eggplant (Solanum melongena L.). In order to generate transgenic resistance against the wilt diseases, Agrobacteriummediated gene transfer was performed to introduce alfalfa glucanase gene encoding an acidic glucanase into eggplant using neomycin phosphotransferase (npt-II) gene as a plant selection marker. The transgene integration into eggplant genome was confirmed by Polymerase chain reaction (PCR) and Southern blot analysis and transgene expression by the glucanase activity and western blot analysis. The selected transgenic lines were challenged with V. dahliae and F. oxysporum under in vitro and in vivo growth conditions, and transgenic lines showed enhanced resistance against the wilt-causing fungi with a delay of 5-7 days in the disease development as compared to wild-type plants.

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“…Further, glucanase is a hydrolytic enzyme, which breaks down the cell wall component, glucan of many necrotrophic fungal pathogens. An increased level of resistance in transgenic potato plants expressing soybean glucanase gene against Phytopthora infestans has been reported due to the increased glucanase activity (Borkowoska et al 1998). Transgenic kiwifruit over-expressing soybean b-1,3 glucanase gene exhibited a six fold increased enzyme activity leading to a decrease in the disease lesion area caused by the gray mold fungus, Botrytis cinerea (Nakamura et al 1999).…”

Section: Disease Resistancementioning

confidence: 99%

Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review

et al. 2017

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Genetic engineering technique offers myriads of applications in improvement of horticultural crops for biotic and abiotic stress tolerance, and produce quality enhancement. During last two decades, a large number of transgenic horticultural crops has been developed and more are underway. A number of genes including natural and synthetic Cry genes, protease inhibitors, trypsin inhibitors and cystatin genes have been used to incorporate insect and nematode resistance. For providing protection against fungal and bacterial diseases, various genes like chitinase, glucanase, osmotin, defensin and pathogenesis-related genes are being transferred to many horticultural crops world over. RNAi technique has been found quite successful in inducing virus resistance in horticultural crops in addition to coat protein genes. Abiotic stresses such as drought, heat and salinity adversely affect production and productivity of horticultural crops and a number of genes encoding for biosynthesis of stress protecting compounds including mannitol, glycine betaine and heat shock proteins have been employed for abiotic stress tolerance besides various transcription factors like DREB1, MAPK, WRKY, etc. Antisense gene and RNAi technologies have revolutionized the pace of improvement of horticultural crops, particularly ornamentals for color modification, increasing shelf-life and reducing post-harvest losses. Precise genome editing tools, particularly CRISPR/Cas9, have been efficiently applied in tomato, petunia, citrus, grape, potato and apple for gene mutation, repression, activation and epigenome editing. This review provides comprehensive overview to draw the attention of researchers for better understanding of genetic engineering advancements in imparting biotic and abiotic stress tolerance as well as on improving various traits related to quality, texture, plant architecture modification, increasing shelf-life, etc. in different horticultural crops.

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Transgenic Potato Plants Expressing Soybean ß-1,3-Endoglucanase Gene Exhibit an Increased Resistance to Phytophthora infestans

Cited by 24 publications

References 0 publications

Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials

Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials

Increased resistance to fungal wilts in transgenic eggplant expressing alfalfa glucanase gene

Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review

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