Background: Cancer is the second cause of death in developed countries. Colon and breast cancers are among the most prevalent ones. Research focusing on finding new natural products with fewer side effects to fight cancer is increasing. Objectives: The present study aimed to evaluate the cytotoxicity of Centaurea albonitens Turrill methanol extract and its fractions against colon and breast cancer cell lines and a normal cell line of bovine kidney cells. Methods: The methanol extract and petroleum ether, chloroform and aqueous fractions were provided from the aerial parts of C. albonitens by maceration method in three days. Each day the solvent was refreshed. Colon (HT-29) and breast (MCF-7) cell lines were treated with the extract/fractions for 48 h for evaluating the cytotoxic activity by MTT assay. The apoptotic induction potential was also evaluated with the Hoechst 33258 staining method. Results: The most considerable effect was reported from the chloroform fraction with IC50 values of 25.6 and 25.1 μg/mL in MCF-7 and HT-29 cells, respectively. In Hoechst staining, condensed chromatin of the apoptotic cells was observed in both cell lines. Conclusion: Centaurea albonites can be suggested for further cancer research studies.
Background & Objectives: Natural products extracted from medicinal plants can play an important role in cancer treatment. This study was designed to investigate antibacterial activity and cytotoxicity of ethanolic extract of Lilium ledebourii (Baker) Boiss bulbs on HeLa cell line. Materials & Methods: In this experimental study, ethanolic extract of Lilium ledebourii bulbs was prepared and its cytotoxicity on HeLa cell line was investigated. Hela cell line was treated with various concentrations of extract (0.156-10.0 mg ml-1) for 72 hours and growth inhibition was assayed using MTT test. In addition, antibacterial activity of the extract was examined using disc diffusion method against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Statistical analysis was performed using SPSS software. Results: The results showed that the ethanolic extract at different concentrations significantly reduced the growth of HeLa cell compared to the control group. The most pronounced growth inhibition was %87.42 at the concentration of 1.25 mg ml-1. In addition, the results indicated that ethanolic extract of Lilium ledebourii bulbs possessed antibacterial activity. Among the aforementioned bacteria, the highest antibacterial activity was seen against S. aureus and the lowest activity against P. aeruginosa. Conclusion: The results of this study revealed that the ethanolic extract of Lilium ledebourii bulbs possesses antibacterial activity and cytotoxicity against Hela cell line. In order to find the underlying mechanism of this activity, further research should be done.
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