Evaluation of antibacterial activity and cytotoxicity of ethanolic extract of Lilium ledebourii bulbs on HeLa cell line

Abstract

Background & Objectives: Natural products extracted from medicinal plants can play an important role in cancer treatment. This study was designed to investigate antibacterial activity and cytotoxicity of ethanolic extract of Lilium ledebourii (Baker) Boiss bulbs on HeLa cell line. Materials & Methods: In this experimental study, ethanolic extract of Lilium ledebourii bulbs was prepared and its cytotoxicity on HeLa cell line was investigated. Hela cell line was treated with various concentrations of extract (0.156-10.0 mg ml-1) for 72 hours and growth inhibition was assayed using MTT test. In addition, antibacterial activity of the extract was examined using disc diffusion method against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Statistical analysis was performed using SPSS software. Results: The results showed that the ethanolic extract at different concentrations significantly reduced the growth of HeLa cell compared to the control group. The most pronounced growth inhibition was %87.42 at the concentration of 1.25 mg ml-1. In addition, the results indicated that ethanolic extract of Lilium ledebourii bulbs possessed antibacterial activity. Among the aforementioned bacteria, the highest antibacterial activity was seen against S. aureus and the lowest activity against P. aeruginosa. Conclusion: The results of this study revealed that the ethanolic extract of Lilium ledebourii bulbs possesses antibacterial activity and cytotoxicity against Hela cell line. In order to find the underlying mechanism of this activity, further research should be done.