The confinement by COVID-19 has meant a re-reading of housing for Spanish households, resulting in the only available and safe space to carry out daily activity. This complex phenomenon has generated a completely different way of inhabiting it, as well as of relating to domestic spaces. For this reason, the home perception and its characteristics must be evaluated, highlighting those perceived as deficiencies, or as preferences in such an unusual context as lockdown, where the experience was different depending on the dwelling characteristics, and the family in question. To deepen in this double perception home-dwelling, a mixed method was used, with two online forms. The first is a quantitative questionnaire, while the second asks the participants for photographs and narratives about such images. More than 1800 surveys and 785 qualitative responses were obtained. From both approaches, the joint discourse arose, allowing an exploratory analysis of the current situation of the Spanish residential park, and the resilience demonstrated in this period by both households and their usual dwellings. This study should facilitate the development of new proposals on housing in contexts similar to the COVID-19 pandemic.

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Pyruvate kinase deficiency (PKD) is a monogenic metabolic disease caused by mutations in the PKLR gene that leads to hemolytic anemia of variable symptomatology and that can be fatal during the neonatal period. PKD recessive inheritance trait and its curative treatment by allogeneic bone marrow transplantation provide an ideal scenario for developing gene therapy approaches. Here, we provide a preclinical gene therapy for PKD based on a lentiviral vector harboring the hPGK eukaryotic promoter that drives the expression of the PKLR cDNA. This therapeutic vector was used to transduce mouse PKD hematopoietic stem cells (HSCs) that were subsequently transplanted into myeloablated PKD mice. Ectopic RPK expression normalized the erythroid compartment correcting the hematological phenotype and reverting organ pathology. Metabolomic studies demonstrated functional correction of the glycolytic pathway in RBCs derived from genetically corrected PKD HSCs, with no metabolic disturbances in leukocytes. The analysis of the lentiviral insertion sites in the genome of transplanted hematopoietic cells demonstrated no evidence of genotoxicity in any of the transplanted animals. Overall, our results underscore the therapeutic potential of the hPGK-coRPK lentiviral vector and provide high expectations toward the gene therapy of PKD and other erythroid metabolic genetic disorders.

The COVID-19 pandemic and the precautionary measures applied globally (lockdowns and curfews) have impacted homes, including work. Working from home (WFH) has emerged as a growing trend in the post-pandemic era. The research question was: Are our homes ready for teleworking? To respond, a national prospective mixed approach was launched for Spanish households during the spring 2020 lockdown, using two online questionnaires, one quantitative and the other qualitative. Through a survey, photographs, and narratives, the study evaluates the perceived adequacy of telework spaces and their specific characteristics, the availability of digital resources and the internet. A total of 1800 surveys and over 200 images and texts related to telework environments were obtained. The results suggest that the adequacy of these spaces was insufficient for more than a quarter of the homes. Also, strong relations between the perceived workspace adequacy and a social status or stability of homes were shown and validated, despite other sociodemographic features, the home composition or habitat were not related. Some other variables statistically significant were occupation regime, type and surface of dwellings; their indoor environmental quality; the availability of exclusive spaces for teleworking; quality of digital resources; and the specific space features. The analysis was completed with qualitative insights through photos and texts. Telework, lived in this context as an experiment, needs this reflection from an environmental, resource-availability, and ergonomic point of view.

We determined the genotype and allelic frequency of several genetic polymorphisms (ACE I/D, GDF-8K153R [and also E164K, P198A and I225T] and AMPD1 C34T) that are candidates to influence sports performance in a group of 54 male professional soccer players. Their results were compared with those of elite endurance male athletes (52 runners) and 123 sedentary, healthy men (controls). We found statistical significance for the ACE ID (chi (2)((2))=8.176, P=0.017) and II genotypes (chi(2)((2))=16.137, P<0.001) with a higher and lower frequency of ID ( P=0.005) and II (P<0.001), respectively, in soccer players than in endurance runners. Statistical significance was also reached for AMPD1 (with a higher frequency of the CT genotype in soccer players than in runners [chi(2)((2))=7.538, P=0.006]) but not for GDF-8 K153R. Since the ACE II genotype is associated with improved potential for endurance performance but with decreased training gains in muscle mass and strength, these findings together with previous results support the notion that elite soccer players tend to have a power/strength oriented genotype.

Following the 2020 confinement due to the COVID-19 pandemic, housing has become the only safe place and this has exposed inequity in habitability. This research on the reality of confined households and the perception of their homes in the Mexican republic is based on a mixed participatory study, combining quantitative and qualitative approaches. The online questionnaire consisted of 58 questions in the quantitative approximation. The qualitative part required the provision of an image of the workspace, with testimonies and personal reflections. During the lockdown, all participants saw an increase in overall energy consumption; more than half reported not being in thermal comfort; and a third declared deficiencies in noise insulation. Regarding the perception of the telework/tele-study space, we found the following categories: bedrooms, living/dining rooms, studies and others. In addition, respondents had often adapted the workspace for both individual and shared use. In general, the households were satisfied with the size of their houses but would like landscaped spaces or better views outside. Confinement made housing the protective element against the pandemic. The consequences will have an effect globally, so new architectural design paradigms need to be rethought.

The pandemic generated by the SARS-CoV-2 virus has led to a forced increase in the number of hours spent at home. In many cases, the lockdown situations, both in social and work terms, have meant that homes have suddenly also become workplaces. Based on all the indicators, this new relational scenario in the labor market displays a clear upward trend and is far from being a temporary situation. It is known that sunlight affects people’s circadian rhythm and that its reduction and even absence during this period of isolation has had a psychological impact on the population. This makes it necessary to reconsider the regulations applied in homes, in order to guarantee their habitability, given their recent widespread use as offices, as well as domestic spaces. In historic centers, the comprehensive renovations being carried out include improvements in energy efficiency and thermal comfort, which play a fundamental role. However, the energy consumption linked to artificial lighting and the quality of this lighting itself have remained in the background, as improvement strategies consist mainly in the replacement of incandescent or fluorescent lamps with LED lamps. Prior to the pandemic, the electric consumption of lighting systems accounted for 10–15% of the total, a figure which increased to 40–50% during the lockdown period. Aiming to improve people’s well-being while reducing energy expenditure on lighting, this article presents a quantitative approach to improving the levels of natural lighting in residential heritage buildings located in historic centers. According to data obtained from previous surveys of a sector of the population, homes built prior to 1950 were characterized by good natural lighting conditions and a very low incidence of health issues among occupants compared to contemporary homes. The objective was to quantify the circadian stimulus and lighting levels and to identify the areas or work areas in homes in order to optimize consumption related to lighting and to generate healthy and comfortable spaces. Results show that historic homes have enough naturally lit areas to perform office work during business hours. However, in the most unfavorable seasons, winter and autumn, it is necessary to use artificial lighting at the start and end of the working day.

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