Sodium benzoate is a permitted food additive by international laws in processing restrictive amounts, but its content must be declared and must not exceed the established limits by legislation. An experimental study was performed by high-performance liquid chromatography (HPLC) for determining the level of sodium benzoate in different brands of soft drinks available in the markets, stores, and shops in the Tangail region of Bangladesh. A Luna 5 µ C18 (2) 100A column (250×4.6 mm) was used for the chromatographic analysis. Chromatographic separation was achieved with an isocratic solvent system comprising sodium acetate and acetic acid buffer (pH = 4.0)/acetonitrile in the ratio of 80:20 (1 mL/min) at 37oC and the chromatograms were recorded at 254 nm. The limit of detection and quantification for sodium benzoate was 0.0000493 mg/100 mL and 0.000149 mg/100 mL, respectively. Quantification of the selected brand soft drinks revealed that the level of the used sodium benzoate was within the FDA standard range. However, by comparing with the Bangladesh Standard and Testing Institute (BSTI), brand -2 and brand-3 soft drink samples were found to exceed the current legal limits.
A reversed-phase high-performance liquid chromatography analysis was carried out for rapid determination and quantification of sodium benzoate in different jelly products. Sodium benzoate is allowed food additive by universal laws in processing in restrictive amounts, but its content must be declared and must not exceed the established limits by legislation. An experimental study for the level of sodium benzoate in different brands of jelly available in the markets, stores and shops in the Tangail region in Bangladesh was determined by high-performance liquid chromatography. Chromatographic separation was achieved with isocratic solvent system of sodium acetate and acetic acid buffer (pH = 4.0): acetonitrile as 70:30 with a flow rate of 0.8 mL/min; chromatograms were recorded at 254 nm. The injection volume was 20 µL. The concentrations of sodium benzoate in all the samples were calculated by the external standard method with calibration of correlation coefficient 0.985 from the standards calibration curves. The limit of detection and quantification for sodium benzoate was 0.0003 mg/100 mL and 0.0009 mg/100 mL respectively. Quantification of the selected brand jellies revealed that the level of the used sodium benzoate was within the FDA standard range. But by comparing with the Bangladesh Standard and Testing Institute (BSTI), Brand-3 jelly samples were found to exceed the current legal limits.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.