Sodium benzoate is a permitted food additive by international laws in processing
restrictive amounts, but its content must be declared and must not exceed the established
limits by legislation. An experimental study was performed by high-performance liquid
chromatography (HPLC) for determining the level of sodium benzoate in different brands
of soft drinks available in the markets, stores, and shops in the Tangail region of
Bangladesh. A Luna 5 µ C18 (2) 100A column (250×4.6 mm) was used for the
chromatographic analysis. Chromatographic separation was achieved with an isocratic
solvent system comprising sodium acetate and acetic acid buffer (pH = 4.0)/acetonitrile in
the ratio of 80:20 (1 mL/min) at 37oC and the chromatograms were recorded at 254 nm.
The limit of detection and quantification for sodium benzoate was 0.0000493 mg/100 mL
and 0.000149 mg/100 mL, respectively. Quantification of the selected brand soft drinks
revealed that the level of the used sodium benzoate was within the FDA standard range.
However, by comparing with the Bangladesh Standard and Testing Institute (BSTI), brand
-2 and brand-3 soft drink samples were found to exceed the current legal limits.
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