The ethanol extracts of Syzygium aromaticum flower bud were tested for anti-nociceptive and antiinflammatory effects in mice and Wistar rats which were carried out using acetic acid-induced abdominal contractions in mice and formalin-induced hind paw edema in Wistar rats. Three doses of the ethanol extract (50, 100, and 200mg/kg body weight i.p.) were used for both studies. The extract had an LD 50 of 565.7 mg/kg body weight intraperitoneally in mice. The extracts produced significant effect (P<0.05) at all the three doses. Similarly, the anti-nociceptive activity produced significant effects (P<0.05) at all the three doses of the extract. The result supports the local use of the plant in painful and inflammatory conditions.

The hypoglycemic effects of aqueous leaves extract of Ocimum gratisimum was investigated in streptozocin-induced diabetic rats. A single administration of the extract at the doses of 250, 500 and 1000 mg/kg body weight was done. The aqueous extract at the dose of 500 mg/kg significantly lowered blood glucose level (P<0.05) of the diabetic rats by 81.3% after 24 h of extract administration. Preliminary phytochemical screening revealed the presence of reducing sugars, cardiac glycosides, resin, tannins, saponins, glycosides, flavonoids, glycerin and steroids. The median lethal dose (LD 50) in rats was calculated to be 1264.9 mg/kg body weight. In conclusion, the leaves extract of O. gratissmm possess antidiabetic in streptozocin-induced in diabetic rats.