Effects of aqueous leaves extract of Ocimum gratissimum on blood glucose levels of streptozocin-induced diabetic wistar rats

Mohammed, A.; Tanko, Y.; Okasha, M. A.; Magaji, R.A.; Yaro, Alpha Seydou

doi:10.5897/ajb2007.000-2323

Cited by 38 publications

(2 citation statements)

References 11 publications

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“…The objective of the present study was to evaluate the effects of OG on sperm quality and testicular cytoarchitecture in male diabetic rats. The reduction in the fasting blood glucose level observed in the diabetic animals treated with OG is consistent with the reported hypoglycemic effect of OG (Aguiyi et al , 2000; Owoyele et al , 2005; Egesie et al , 2006; Mohammed et al , 2007; Oguanobi et al , 2012; Okoduwa et al ., 2017). Fructosamine was quantified in the present study to monitor glycemic control in the diabetic rats.…”

Section: Discussionsupporting

confidence: 87%

Ocimum gratissimum leaf extract may precipitate infertility in male diabetic Wistar rats

Shittu

Olatunji

et al. 2019

JBRA Assisted Reproduction

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Objective:This study was designed to investigate the Ocimum gratissimum (OG) effects on sperm quality and testicular cytoarchitecture in alloxan-induced diabetic rats.Method:Twenty male Wistar rats (150-200 g) were assigned into 4 groups (n=5) as A (control), B (OG), C (Dia) and D (Dia+OG). Groups A and B were normal animals receiving distilled water or OG (400 mg/kg), respectively while diabetes was induced by alloxan monohydrate (100 mg/kg) in groups C and D, followed by the administration of distilled water or OG, respectively for 28 days. Blood samples were obtained for fasting blood glucose (FBG) and fructosamine determination while, epididymis and testes were obtained for sperm quality assessment using computer-assisted sperm analyzer and testicular histomorphometry, respectively. Seminiferous tubule diameter and interstitial space distance were quantified in hematoxylin and eosin stained slides. Statistical analysis was done using ANOVA and student t-test at α0.05.Results:Fructosamine and FBG were reduced in Dia+OG (80.11±3.80µmol/L and 132.0±8.41mg/dl, respectively) compared with Dia (139.66±4.29µmol/L and 285.6±26.69mg/dl, respectively). Sperm count was unchanged in Dia, but decreased in OG and Dia+OG; abnormal sperm cells increased in OG, Dia and Dia+OG. Mild vacuolation in the seminiferous tubule, disorganized germinal cells layer, arrested sperm maturation with empty spermatozoa in lumen, decreased seminiferous tubule diameter and increased interstitial space were found in the testes of OG, Dia and Dia+OG compared with control.Conclusion:Diabetes induces sperm impairments and distortions in testicular cytoarchitecture, which were aggravated by OG leaf extract in male Wistar rats.

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Section: Discussionsupporting

confidence: 87%

Ocimum gratissimum leaf extract may precipitate infertility in male diabetic Wistar rats

Shittu

Olatunji

et al. 2019

JBRA Assisted Reproduction

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“…Its leaves possess volatile essential oil which comprises majorly about 31%–66% thymol, and eugenol. It also has xanthones, terpene and lactone ( Ezekwesili et al, 2004 ; Mohammed et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Reversal of mitochondrial permeability transition pore and pancreas degeneration by chloroform fraction of Ocimum gratissimum (L.) leaf extract in type 2 diabetic rat model

Salemcity,

Olanlokun,

Olowofolahan

et al. 2023

Front. Pharmacol.

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Introduction: Unmanaged Diabetes Mellitus (DM) usually results to tissue wastage because of mitochondrial dysfunction. Adverse effects of some drugs used in the management of DM necessitates the search for alternative therapy from plant origin with less or no side effects. Ocimum gratissimum (L.) (OG) has been folklorically used in the management of DM. However, the mechanism used by this plant is not fully understood. This study was designed to investigate the effects of chloroform fraction of OG leaf (CFOG) in the reversal of tissue wastage in DM via inhibition of mitochondrial-mediated cell death in streptozotocin (STZ)-induced diabetic male Wistar rats.Methods: Air-dried OG leaves were extracted with methanol and partitioned successively between n-hexane, chloroform, ethylacetate and methanol to obtain their fractions while CFOG was further used because of its activity. Diabetes was induced in fifteen male Wistar rats, previously fed with high fat diet (28 days), via a single intraperitoneal administration of STZ (35 mg/kg). Diabetes was confirmed after 72 h. Another five fed rats were used as the normal control, treated with corn oil (group 1). The diabetic animals were grouped (n = 5) and treated for 28 days as follows: group 2 (diabetic control: DC) received corn oil (10 mL/kg), groups 3 and 4 were administered 400 mg/kg CFOG and 5 mg/kg glibenclamide, respectively. Body weight and Fasting Blood Glucose (FBG) were determined while Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) and beta cell (HOMA-β), and pancreatic tissue regenerating potential by CFOG were assessed. Activity-guided purification and characterization of the most active principle in CFOG was done using chromatographic and NMR techniques. The animals were sacrificed after 28 days, blood samples were collected and serum was obtained. Liver mitochondria were isolated and mitochondrial permeability transition (mPT) was investigated by spectrophotometry.Results: CFOG reversed diabetic-induced mPT pore opening, inhibited ATPase activity and lipid peroxidation. CFOG reduced HOMA-IR but enhanced HOMA-β and caused regeneration of pancreatic cells relative to DC. Lupanol was a major metabolite of CFOG.Discussion: Normoglycemic effect of CFOG, coupled with reversal of mPT, reduced HOMA-IR and improved HOMA-β showed the probable antidiabetic mechanism and tissue regenerating potentials of OG.

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Aromatic Plants: Use and Nutraceutical Properties

Landi

2014

Novel Plant Bioresources

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Effects of aqueous leaves extract of Ocimum gratissimum on blood glucose levels of streptozocin-induced diabetic wistar rats

Cited by 38 publications

References 11 publications

Ocimum gratissimum leaf extract may precipitate infertility in male diabetic Wistar rats

Ocimum gratissimum leaf extract may precipitate infertility in male diabetic Wistar rats

Reversal of mitochondrial permeability transition pore and pancreas degeneration by chloroform fraction of Ocimum gratissimum (L.) leaf extract in type 2 diabetic rat model

Aromatic Plants: Use and Nutraceutical Properties

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