Many fermentation volatiles important to wine aroma potentially arise from yeast metabolism of hexose sugars, but assessing the relative importance of these pathways is challenging due to high endogenous hexose substrate concentrations. To overcome this problem, gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) was used to measure high-precision 13C/12C isotope ratios of volatiles in wines produced from juices spiked with tracer levels (0.01–1 APE) of uniformly labeled [U-13C]-glucose. The contribution of hexose to individual volatiles was determined from the degree of 13C enrichment. As expected, straight-chain fatty acids and their corresponding ethyl esters were derived almost exclusively from hexoses. Most fusel alcohols and their acetate esters were also majority hexose-derived, indicating the importance of anabolic pathways for their formation. Only two compounds were not derived primarily from hexoses (hexanol and isobutyric acid). This approach can be extended to other food systems or substrates for studying precursor–product relationships.
BackgroundSlowly digested carbohydrates are perceived as beneficial by some consumers, and various regulatory bodies have published specific criteria defining lower postprandial glycemic response. We developed an optimized savory cluster snack containing slowly digested starch.ObjectiveWe compared the glucose and insulin responses elicited by the optimized (test-) cluster, a control-cluster, and an available-carbohydrate-matched portion of white bread in healthy individuals. The primary outcome was blood-glucose peak rise.We tested healthy individuals (n = 25) on 3 occasions using a randomized crossover design. On each occasion, the participants provided fasting blood samples and then consumed 1 serving of test-cluster, control-cluster, or white bread. We then measured the participants’ blood-glucose and serum-insulin concentrations over the next 4 h.ResultsThe test-cluster elicited a significantly lower blood-glucose peak rise (mean ± SEM: 1.24 ± 0.09 mmol/L) and incremental area under the curve (iAUC; 67 ± 8 mmol × min/L) than the control-cluster (2.27 ± 0.13 mmol/L and 117 ± 10 mmol × min/L, respectively) and white bread (2.27 ± 0.16 mmol/L and 114 ± 9 mmol × min/L, respectively). The serum-insulin peak rise and iAUC elicited by the test-cluster (128 ± 13 pmol/L and 6.10 ± 0.73 nmol × min/L, respectively) and white bread (141 ± 20 pmol/L and 6.47 ± 1.11 nmol × min/L, respectively) were significantly lower than those elicited by the control-cluster (205 ± 26 pmol/L and 9.60 ± 1.31 nmol × min/L, respectively).ConclusionThe test-cluster elicited lower glucose and insulin responses than the control-cluster. The results support the hypothesis that the carbohydrates in the test-cluster are digested and absorbed slowly in vivo.
This study compared the effect of a snack with ingredients to slow carbohydrate digestion (Test-snack) on postprandial blood glucose and insulin concentrations and subjective appetite ratings. We hypothesized that Test-snack would lower glucose and insulin responses and reduce appetite compared with a Control-snack. Overweight or obese subjects (n = 17) completed a randomized crossover study. Glucose, insulin, and appetite ratings were measured before consuming each snack or white bread (Bread) and over a period of 4 h. Subjects received Test-snack, Control-snack, or Bread in random order at least a week apart. The a priori primary outcome was the glucose response, and the secondary outcomes were appetite ratings and insulin responses. Mixed effects statistical models were used to perform analysis of variance in terms of the area under curve (AUC) and at specific time points. The 2-h AUC for glucose was significantly lower with Test-snack compared to Control-snack and Bread
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