Goat ovaries were collected from the slaughterhouse and categorized as right, left, corpus luteum (CL)-present and -absent group and evaluated on the basis of weight (g), length (cm), width (cm), number of follicles, follicles aspirated and number and state of cumulus-oocyte-complexes (COCs). Comparatively higher weight [(0.66+/-0.02) vs (0.64+/-0.02) g], length [(1.17+/-0.02) vs (1.11+/-0.02) cm] and width [(0.77+/-0.02) vs (0.74+/-0.02) cm] were found in right ovaries than those of left. On the other hand significantly (P<0.05) higher weight [(0.71+/-0.03) vs (0.64+/-0.01) g] and width [(0.76+/-0.03) vs (0.75+/-0.01) cm] were found in CL-present group than those of CL-absent group of ovaries. The left ovaries contained comparatively higher number of normal COCs [(1.06+/-0.09) per ovary] than right ovaries [(1.03+/-0.10) per ovary] and the similar trend was found in total number of follicles [(4.51+/-0.25) vs (4.30+/-0.23) per ovary] and follicles aspirated [(2.55+/-0.14) vs (2.52+/-0.12) per ovary]. But the total COCs per ovary was almost similar in both ovaries [right and left: (1.85+/-0.12) and (1.85+/-0.11) per ovary, respectively]. Higher number of total COCs [(1.87+/-0.09) vs (1.76+/-0.16) per ovary], total number of follicles [(4.45+/-0.19) vs (4.16+/-0.37) per ovary], follicles aspirated [(2.55+/-0.10) vs (2.48+/-0.21) per ovary] and normal COCs [(1.12+/-0.07) vs (0.76+/-0.14) per ovary] were found in CL-absent group than those of CL-present group of ovaries.
Abstract. Selenium (Se) and vitamin E (Vit-E), as integral parts of antioxidant systems, play important roles for sperm and embryos in vitro. In this study, the effects of Se and Vit-E on the maturation, in vitro fertilization and culture to blastocysts of porcine oocytes and accumulation of ammonia in the culture medium during different development stages were investigated. The maturation was performed in modified tissue culture medium (mTCM)-199 supplemented with 10% (v/v) porcine follicular fluid, the fertilization medium was modified Tyrode's albumin lactate pyruvate (mTALP), and the embryo culture medium was modified North Carolina State University (mNCSU)-23. Se in the form of sodium selenite (SS) and seleon-L-methionine (SeMet) and Vit-E at different concentrations were also used. The incorporation and oxidation of 14 C(U)-glucose were assessed with a liquid scintillation counter. In this study, SeMet and SeMet+Vit-E increased oocyte maturation, fertilization and incorporation and oxidation of 14 C(U)-glucose significantly (P<0.05) compared with the control and other treatments. In addition, embryo development, specifically in terms of the numbers of morulae and blastocysts, significantly increased (P<0.05) with SeMet and SeMet+Vit-E. In contrast, the accumulation of ammonia was reduced with SeMet and SeMet+Vit-E compared with other treatments. These findings indicate that SeMet and SeMet+Vit-E may play important roles in reducing the accumulation of ammonia and subsequently in increasing the rate of maturation of porcine oocytes and fertilization, as well as development of the blastocyst and utilization of glucose in in vitro maturation, fertilization and culture to blastocysts of porcine oocytes.
This experiment was undertaken to know the phenotypic characteristics of native sheep. The study was conducted to investigate morphometric characters of sheep in three different field sites (Naogaon, Noakhali and Tangail) and Bangladesh Livestock Research Institute (BLRI) nucleus flock through close observation, measuring and record keeping. Characteristics of body weight, body length, heart girth, wither height, fore leg length, hind leg length, head length, ear length, ear width, horn length etc were collected from mature sheep. The results indicate that the average litter size, birth weight, weaning weight and body weight gain were comparatively higher in sheep of BLRI nucleus flock than those of other three regions. The average numbers of service/conception, post partum heat period (days), age at first heat (days), age at first lambing (days), lambing interval (days) were 1. 30, 34.13, 266.50, 432.72, 192.17 respectively in BLRI nucleus flock which were comparatively better than the other three regions. The average litter size, birth weight (kg), weaning weight (kg) and body weight gain (g) in nucleus flock were 1.80, 1.19, 6.74 and 60.70 consequently. The overall performance of the sheep of BLRI nucleus flock were comparatively better than the others which may be due to the result of selective breeding, improve feeding, housing, health management etc.
Characterization of Black Bengal bucks and does was performed in this study. A total of 50 goats of different aged were studied. The experiment was performed in Black Bengal bucks and does at 1 st day and at 3, 6, 9 and 12 months of age for measuring different parameters such as body length, heart girth, wither height, hip height, fore and hind leg length, head length and width, head length and width, ear length and ear breath, scrotal length and scrotal circumference. In a single parameter as body weight of Black Bengal bucks and does at 1 st day and at 3, 6, 9 and 12 months of age were found to be 1.08±0.06, 5.22±0. 33, 8.95±0.34, 12.05±0.47 and 14.20±0.20; and 1.01±0.23, 3.93±0.19, 7.41±0.27, 9.53±0.38 and 12.40±0.41 kg respectively. Whereas all the finding where gradually increased for all parameters according to their specific age periods. Body weight has a strong positive relationship with heart girth, body length, height at wither, scrotal length and scrotal circumference in Black Bengal goat. All the parameters studied found comparatively higher in bucks than does.
The present study was conducted with a view to know the productive and reproductive parameters of
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