Selenium and Vitamin E Improve the <i>In Vitro</i> Maturation, Fertilization and Culture to Blastocyst of Porcine Oocytes

Abstract

Abstract. Selenium (Se) and vitamin E (Vit-E), as integral parts of antioxidant systems, play important roles for sperm and embryos in vitro. In this study, the effects of Se and Vit-E on the maturation, in vitro fertilization and culture to blastocysts of porcine oocytes and accumulation of ammonia in the culture medium during different development stages were investigated. The maturation was performed in modified tissue culture medium (mTCM)-199 supplemented with 10% (v/v) porcine follicular fluid, the fertilization medium was modified Tyrode's albumin lactate pyruvate (mTALP), and the embryo culture medium was modified North Carolina State University (mNCSU)-23. Se in the form of sodium selenite (SS) and seleon-L-methionine (SeMet) and Vit-E at different concentrations were also used. The incorporation and oxidation of 14 C(U)-glucose were assessed with a liquid scintillation counter. In this study, SeMet and SeMet+Vit-E increased oocyte maturation, fertilization and incorporation and oxidation of 14 C(U)-glucose significantly (P<0.05) compared with the control and other treatments. In addition, embryo development, specifically in terms of the numbers of morulae and blastocysts, significantly increased (P<0.05) with SeMet and SeMet+Vit-E. In contrast, the accumulation of ammonia was reduced with SeMet and SeMet+Vit-E compared with other treatments. These findings indicate that SeMet and SeMet+Vit-E may play important roles in reducing the accumulation of ammonia and subsequently in increasing the rate of maturation of porcine oocytes and fertilization, as well as development of the blastocyst and utilization of glucose in in vitro maturation, fertilization and culture to blastocysts of porcine oocytes.

Effect of Dipeptides on &lt;italic&gt;In vitro&lt;/italic&gt; Maturation, Fertilization and Subsequent Embryonic Development of Porcine Oocytes

“…The incorporation and oxidation of glucose serve as indicators of the vitality of oocytes and the resulting eggs (Tsujii et al, 2009). In this study, the incorporation and oxidation rates of 14 C(U)-glucose were significantly higher in AlaGln+GlyGln treated oocytes than in the control group during maturation, fertilization, and the 2-cell and blastocyst stages, which was in agreement with the results of our previous study (Tareq et al, 2012). We reported that the combination of selenium and vitamin E in comparison with single supplementation improves porcine embryos, which suggests that the incorporation and oxidation rates of 14 C(U)-glucose were higher in embryos treated with SeMet and SeMet+Vitamin-E at the 2-cell and blastocyst stages.…”

“…It has been suggested that Gln could be replaced with L-alanyl-L-glutamine (AlnGln) and L-glycyl-L-glutamine (GlyGln) to achieve an increased level of embryonic deployment in mice (Biggers, 2004). We previously reported that the accumulation of ammonia in the medium could be reduced by supplementation with seleon L-methionine (SeMet) and SeMet+Vitamin-E (Tareq et al, 2012). Dipeptides play an important role in oocyte maturation (Tareq et al, 2007) and the porcine sperm acrosome reaction (Tareq et al, 2008).…”

Dual effect of fetal bovine serum on early development depends on stage-specific reactive oxygen species demands in pigs

“…Proportion of TE cells in porcine IVP blastocysts, compared to in vivo blastocysts, are approximately 46% and 60%, respectively, [27, 28]. Thus, many researchers have attempted to reduce oxidative stress levels by adding numerous supplements with ROS scavenging activity, such as anthocyanin [29], L-carnitine [30], hypotaurine [31], vitamin C [32], β-mercaptoethanol [33, 34] and selenium [35]. Although the effects of FBS supplementation on early development of mammalian IVP embryos have been reported, to our knowledge, no evidence has been published regarding the relationship between FBS and oxidative stress during the early development of mammalian embryos.…”

Effect of 7,8-Dihydroxyflavone as an Antioxidant on <i>In Vitro</i> Maturation of Oocytes and Development of Parthenogenetic Embryos in Pigs

“…Therefore, many studies have been performed to reduce ROS using antioxidant treatments such as anthocyanin [18], L-carnitine [19], hypotaurine [20], vitamin C [21], β-mercaptoethanol [22, 23] and Selenium [24]. 7,8-Dihydroxyflavone (7,8-DHF), a kind of flavonoid (Fig.…”