Food-borne pathogens causing infections and intoxications can affect everyone. Escherichia (E) coli is one of the major food borne bacterial pathogens. This study was conducted to investigate the prevalence of E. coli in milk, chicken meat and beef and to determine the multi-drug resistance profile of E. coli in Mymensingh district, Bangladesh. A total of 169 samples including milk (n=108), chicken meat (n=51) and beef (n=10) were collected from Bangladesh Agricultural University (BAU) dairy farm, American dairy farm, Gazipur and retail markets of municipal area during July 2016 to June 2017. E. coli were isolated and identified by colony characteristics on selective agar like Eosine-methylene blue (EMB) agar, Salmonella-Shigella (SS) agar, Gram staining, biochemical test and Polymerase Chain Reaction (PCR). The overall prevalence of E. coli in all food samples was 37.86%. A total of 32 (29.63%) milk, 25 (49.02%) chicken meat and 07 (70%) beef samples were E. coli positive through conventional method. Among 64 samples only 23 samples (35.94%) were confirmed by PCR. Multi-drug resistant E. coli were detected by disc diffusion test using 10 commonly used antibiotics. Antibiogram study showed that E. coli isolated from chicken meat were resistant to oxytetracycline (92%), sulphonamide-trimethoprim (84%), amoxycillin (76%) and erythromycin (60%). E. coli isolated from beef sample were resistant to erythromycin (85.71%) and oxytetracycline (71.43%) and sensitive to ciprofloxacin (100%), gentamicin (100%) and neomycin (100%). However, all isolates of E. coli were found sensitive to amikacin (100%). E. coli isolated from milk sample were 100% sensitive to gentamicin followed by neomycin, ciprofloxacin, azithromycin, oxytetracycline and erythromycin. Overall 50% of E. coli isolates of food were found multi-drug resistant. About 28.13%, 57.14% and 76% of the E. coli isolates originated from milk, beef and chicken meat respectively were multi-drug resistant. The higher prevalence of E. coli in chicken meat, beef and milk indicates unhygienic production and processing of these foods. Presence of multi-drug resistant E. coli in these foods might pose serious public health threats. The antibiogram profile of the isolates will help therapeutic decision making in the treatment of colibacillosis in cattle and poultry in Bangladesh.

This study was conducted to investigate the prevalence of Salmonella spp. in milk, chicken meat and beef and to determine the multi-drug resistance (MDR) profile of Salmonella spp. in Mymensingh and Gazipur districts, Bangladesh. A total of 169 samples of milk (n=108), chicken meat (n=51) and beef (n=10) were collected from Bangladesh Agricultural University (BAU) dairy farm, American dairy farm, Gazipur and different  small dairy farms of municipal area during July 2016 to June 2017. Salmonella spp. were isolated on various selective agar media such as: Salmonella-Shigella (SS) agar, Xylose-Lysine Deoxycholate (XLD) agar, Eosine-Methylene Blue (EMB) agar. Identification of Salmonella spp. was done by colony characteristics, Gram staining, biochemical test and Polymerase Chain Reaction (PCR). Multi-drug resistant Salmonella spp. was detected by disc diffusion test using 10 commonly used antibiotics. The overall prevalence of Salmonella spp. in all food samples was 21.89%. A total of 29 (56.86%) chicken meat, 02 (1.85%) milk, and 06 (60%) beef samples were Salmonella spp. positive. Antibiogram study showed that an overall 89.19% of Salmonella spp. was found multi-drug resistant. Specifically 100%, 66.67% and 93.10% of the Salmonella spp. isolates originated from milk, beef and chicken meat respectively were multi-drug resistant. The result of this study suggests that MDR Salmonella spp. is prevalent in the milk and meat which might cause public health hazard if proper hygienic measures are not undertaken at farm and marketing level.

The study was intended for molecular detection of E. coli isolated from raw cow's milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying staining characteristics, cultural properties on different selective media, biochemical tests, catalase and coagulase test, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found positive for E. coli. 15 Escherichia coli isolates were amplified by 16S rRNA gene based PCR. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the E. coli isolates were resistant to amoxycillin (86.67%) and erythromycin (73.33%) but sensitive to azithromycin (53.33%), ciprofloxacin (86.67%), gentamicin (86.67%), norfloxacin (80%) and streptomycin (66.67%).

The study was intended for molecular detection of S. aureus isolated from raw cow's milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying cultural properties on different selective media, biochemical tests, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found to be positive for S. aureus. S. aureus specific 16S rRNA gene was amplified from all isolates and identified as S. aureus. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the S. aureus isolates were resistant to amoxicillin (100%), erythromycin (73.33%) and tetracycline (73.33%) but sensitive to azithromycin (93.33%), ciprofloxacin (93.33%), gentamicin (100%), norfloxacin (86.67%) and streptomycin (86.67%).

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