En el presente estudio se realizó la evaluación de la actividad antioxidante mediante el secuestro de radicales libres del DPPH de los frutos de anona, castaña, chope, huasaí, huito y uvilla, frutos originarios de la cuenca amazónica. Así mismo, se determinó la concentración de compuestos fenólicos por espectrofotometría y ácido ascórbico por cromatografía de HPLC. La mayor actividad antioxidante se da en las cáscaras del chopé con IC de 63.02 ug/ml. 50En cuanto a compuestos polifenólicos, presentó mayor concentración la cáscara y pulpa de huito 137.15 y 97.78 mg/100g respectivamente. En tanto que en ácido ascórbico están presentes en altas concentraciones en la pulpa de anona con 4.28 mg/100g y semilla de castaña con 3.33 mg/100g.PALABRAS CLAVE: frutos amazónicos, antioxidantes, ácido ascórbico. EVALUATION OF ANTIOXIDANT ACTIVITY OF SIX AMAZONIAN FRUITS: ANONA, BRAZIL NUT, CHOPE, ACAI BERRY, HUITO AND UVILLA.The antioxidant activity of six Amazonian fruits (anona, brazil nut, chope, acai berry and uvilla) were evaluated by measuring the sequestration of free radicals in DPPH. Additionally, the concentration of phenolic compounds was measured using a spectrophotometer and ascorbic acid was measured using chromatography of HPLC. The peel of chopé has the highest antioxidant activity with IC of 63.02 ug/ml. The highest concentration of phenolics was 50 found in huito, both in the peel and in the pulp (137.15 and 97.78 mg/100g, respectively.) The highest ascorbic acid concentrations were in anona (4.28 mg/100g) and brazil nut (3.33 mg/100g).
Objectives:The present study evaluates novel cationic quinoline derivatives known as benzimidazo[3,2-a]quinolinium salts (BQS) named NBQ-48 and ABQ-48 that have structural similarities to known anti-cancer substances such as ellipticine and berberine.Methods:Toledo human lymphoma (ATCC CRL2631) cells were treated for 24 to 48 hours. Apoptosis related endpoints such as cell cycle arrest, mitochondrial damage, RNS and ROS generation and the activity of several apoptosis related proteins such as caspases and apoptosis inducing factor (AIF) were studied using fluorescence staining and western blot respectively.Results:Results indicated a higher toxicity from the amino substituted ABQ-48 versus the NBQ-48 (GI50’s of 50uM versus 100uM respectively). Both compounds induced cell death through various apoptosis related endpoints including a decrease in mitochondrial membrane potential with an increase in ROS and activation of the effector caspase 3. Interestingly, AIF release was observed on cells treated with the amino substituted ABQ-48 but not on the nitro substituted NBQ-48 samples suggesting a caspase independent mechanism for ABQ-48.Conclusions:The results obtained presents the toxic effects of two novel benzimidazo[3,2-a]quinolinium salts in human lymphoma tumor cells. The identified mechanism of action includes multiple apoptosis related effects. Furthermore the data presents a clear variation in caspase dependent or independent mechanism for each compound.
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