Age-related macular degeneration (AMD) is resulted from choroidal neovascularization (CNV)mediated cicatrization and vision loss. The sustained retinal hypoxia in retinal pigment epithelium (RPE) cells was reported to contribute to CNV. However, the underlying genetic regulatory network of hypoxia response in RPE is not fully understood. In this study, human ARPE-19 RPE cells were cultured under the anoxia for 24 h and later re-oxygenated in normoxia. Then the transcriptome was investigated via high throughput sequencing. We observed that long noncoding RNA (lncRNA) histone deacetylase 4 antisense RNA 1 (HDAC4-AS1) was increased in hypoxic condition compared to normal control and decreased after re-oxygenation addition, while the change of HDAC4 expression was reduced in hypoxic condition compared to normal control and up-regulated after re-oxygenation addition in ARPE-19 cells. Furthermore, HDAC4-AS1 knockdown could suppress the transcription activity of HDAC4 only in hypoxia condition, and fluorescence in situ hybridization and pull down assay indicated that transcripts of HDAC4-AS1 could substantially bind to the promoter of HDAC4 and facilitate the recruitment of HIF-1α. Finally, we also determined the specific regions of HDAC4-AS1 that contribute to the interaction with HIF-1α and the promoter of HDAC4. Taken together, these outcomes declared that HDAC4-AS1 could inhibit HDAC4 expression through regulating HIF-1α in human ARPE-19 cells with hypoxic stress.
In recent years, the concepts and practices of smart cities and “Internet plus” smart energy have mushroomed, greatly promoting the development and application of customer-side energy Internet business and stimulating users’ diversified and personalized demand for interactive services of intelligent power utilization. Intelligent power utilization, as the core of the interactive service system, serves as a bridge between power supply departments and customers. Its construction has a direct impact on the efficiency, economic operation and orderly electricity consumption of power grids. In the context of the increasingly intelligent power grid and complex load structure, this paper analyzed the development trend of energy Internet and adopted SWOT to reveal the strengths, weaknesses, opportunities and challenges of power grid enterprises in developing energy Internet, explore their development ideas on energy Internet businesses, and facilitate their development of energy Internet.
This article builds a model of energy-city development correlation, and factors for energy systems to drive city development. It also analyzes the corresponding indicators and determines the intercorrelation between the indicators. In the end, the article collects and categorizes energy, industry, environment, and other representative indicators from prefecture-level cities in China, and analyzes the factors affecting energy and city development based on the panel data regression model.
In recent years, the energy revolution has become a key in city development and leading economy. This article analyzes the current situation of energy development and city. Firstly, this article analyzes four aspects of problem of coordinated development. Then, this article has classified the city into two major systems. Finally, the correlation between energy and city is analyzed. Not only energy acts as a breakthrough in city development, but also the evolution of urban spatial form affects energy supply patterns.
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