No abstract

Terpenoids are of great interests in a broad range of health-beneficial biological activities and various industrial applications. In plants, terpenoids are synthesized by two distinct pathways, methylerythritol phosphate (MEP) and mevalonate pathways in a separate location. MEP pathway supplies isoprene precursors isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP) of terpenoid biosynthesis in plant plastids. The MEP pathway has been an engineering target to increase the metabolic flux towards higher terpenoid production in plants. 1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) is the terminal step of the MEP pathway to regulate the terpenoid biosynthesis and is encoded by three paralogous genes in Ginkgo biloba. In this study, we assessed the effect of overexpression of GbHDR1 on terpenoid metabolism in poplar plants. Overexpression of GbHDR1 in poplar plants accelerated growth and delayed winter-bud formation. Transcript levels of gibberellin, chlorophylls, and carotenoid biosynthetic genes in GbHDR1-overexpressing (GbHDR1ox) poplars were up-regulated, suggesting metabolic flux enhancement. Moreover, enhanced contents of chlorophylls and carotenoids in the leaves of the GbHDR1ox plants resulted in a higher photosynthetic rate as a consequence. Therefore, we expect the GbHDR1 overexpression will be a desirable engineering point of the MEP pathway for enhancing terpenoid metabolic flux and production in plants.

The quality of the drinking water source reservoirs has always been a research hotspot. However, few studies focused on the water quality of reservoirs over a relatively long period with time series analysis. In this paper, based on water quality and hydrological data from 2010 to 2020, considering 8 water quality parameters, CCME-WQI with time series analysis was used to explore the interannual and seasonal changes in water quality in the Weishui Reservoir. Furthermore, the main factors affecting water quality were discussed through correlation analysis. ARIMA model is used to predict water quality in the future. The results show that the water quality was seriously polluted from 2012 to 2013. After 2018, the water quality gradually improved and stabilized. In addition, the water quality is affected by inflow, showing the characteristics of poor water quality in summer and winter. The key parameters affecting water quality are TN and TP, which are almost 2 times higher than the grade II standard of water quality standard. Through the ARIMA model, it is predicted that CCME-WQI is maintained at 80.46 indicating that the water quality will be stable in the future.