1,2-Limonene oxide was synthesized for the first time via epoxidation reactions of (R)-(+)-limonene mediated by a new immobilized preparation with low-cost material from Candida antarctica lipase fraction B (NS 88011). Parameters affecting this reaction were studied, such as enzyme amount, acyl donor concentration, oxidant concentration, (R)-(+)-limonene concentration, reaction time, and temperature. The synthesis was maximized in fed-batch reactors, considering 40 mM (R)-(+)-limonene, 70 mM octanoic acid, and 250 mM H2O2 (35% aqueous solution). NS 88011 and Novozym 435 led to similar results, which makes NS 88011 a promising and low-cost alternative in epoxidation reactions mediated by lipase. The best result in the 1,2-limonene oxide synthesis was 74.92 ± 1.12% of yield at 40 min. In addition, both NS 88011 and Novozym 435 can be used for up to three cycles. Therefore, NS 88011 had a good and viable performance in the epoxidation reactions of the (R)-(+)-limonene and after purification led to a final product with purity >95%.

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