Enhanced production of surfactin using cassava wastewater and hydrophobic inducers: a prospection on new homologues

Toward Effects of Hydrophobicity on Biosurfactant Production by Bacillus subtilis Isolates from Crude-Oil-Exposed Environments

“…Comparably to the hexadecane supplementation approach, a lower amount of surfactin was produced compared to the control cultivation. This observation is contrary to popular opinion, as it was previously reported that the productivity of biosurfactants could be significantly enhanced by supplementing the culture medium with hydrophilic and/or hydrophobic inducers, such as vegetable oils [26]. Furthermore, it was observed that the specific surfactin production was further reduced by a higher addition of polystyrene beads (Table 2).…”

“…Furthermore, it was observed that the specific surfactin production was further reduced by a higher addition of polystyrene beads (Table 2). Interestingly, other studies described that palmitic acid as a hydrophobic inducer led to the highest yield of surfactin by Bacillus subtilis ATCC 6633 [26], while olive oil was applied as an effective inducer to increase biosurfactant production [27,29] and animal fat was successfully utilized for improving biosurfactant production [71]. Although stimulatory effects on biosurfactant production are described in these previous studies, the addition of oils and fatty acids might affect the metabolic pathways of the respective production strains without having an immediate effect on the basis of hydrophobicity.…”

“…One strategy for improved biosurfactant production is the use of inducers. Hydrophobic inducers serve as additional carbon sources and also trigger biosurfactant production, resulting in the greater solubilization of hydrophobic carbon sources [25][26][27]. For instance, olive oil was used as an effective inducer for the production of biosurfactants [27][28][29].…”

“…Using a combination of carbohydrates and long-chain fatty acids as an inducer, rhamnolipid production was improved [30,31]. In the case of surfactin, Olivera Schmidt et al [26] showed that palmitic acid led to the highest performance among several hydrophobic inducers.…”

Fed-Batch Bioreactor Cultivation of Bacillus subtilis Using Vegetable Juice as an Alternative Carbon Source for Lipopeptides Production: A Shift towards a Circular Bioeconomy

“…Sucrose was the most rapidly metabolized sugar, as can be observed in the media at 90 and 70% VJ. It is indeed completely consumed within 12 h, even during the lag, and this trend is confirmed by Nitschke et al, who found in cassava water a more rapid consumption of sucrose than of fructose and glucose by B. subtilis, going from 20.0 to 1.13 g/L in 12 h [41]; similarly, in another study, it was observed that in the presence of both glucose and sucrose, B. subtilis consumes the latter faster [42].…”