No abstract

Mammary tumours (MT) are one of the most prevalent malignancies in female dogs and women. Currently, molecular analyzes have shown that each tumour type presents its own genetic signature. In this context, liquid biopsy allows a comprehensive genetic characterisation of the tumour, enabling early diagnosis and personalised treatment of patients. In women, deleterious mutations inherited in BRCA2 gene are associated with an increased risk of breast cancer, resistance to therapies and worse prognosis. In female dogs, there are many divergent data on the involvement of BRCA2 gene with mammary carcinogenesis and what its pathogenic potential is. Therefore, the objective was to identify BRCA2 gene variants in 20 plasma DNA samples, from 10 newly diagnosed dogs with mammary cancer (RD), five control (CTR) and five mastectomized patients. Eleven single nucleotide polymorphisms (SNPs) were detected, most of them in the exon 11 and two indels (deletion/insertion) in the BRCA2 gene. However, there was no statistically significant difference in the SNPs/indels detected between the groups. In addition, only one SNP (p.T1425P) and one deletion (p.L2307del) were considered deleterious using in silico computational models. Interestingly, most common variants were present in the plasma of all groups, except for the Ile2614Thr, Ile2614Val, Thr1425Pro and p.L2307del variants. Thus, we observed that SNPs are common in the BRCA2 gene of female dogs with MT, with a similar condition identified in women with breast cancer. Liquid biopsy approach in dogs with MT is useful for genetic and therapeutic proposals.

No abstract

Two lineages of Rhipicephalus sanguineus are known in Brazil: the temperate or southern and the tropical or northern populations. The distribution patterns of both lineages of R. sanguineus have epidemiological implications that can affect vectorial competence concerning Ehrlichia canis, the agent of canine monocytic ehrlichiosis. Intending to identify the microbiomes of both lineages and compare microorganisms in R. sanguineus, we used the 16S rRNA (V4-V5 region) gene-based metataxonomic approach, through NGS sequencing on the MiSeq Illumina platform. We selected specimens of females from the environment and samples of primary embryonic cell cultures, from both lineages, and this was the first study to investigate the prokaryotic microbiome in tick cell cultures. The results showed that many bacterial taxa detected in the samples were typical members of the host environment. A significant diversity of microorganisms in R. sanguineus females and in embryonic cell cultures from both lineages was found, with emphasis on the presence of Coxiella in all samples, albeit in different proportions. The Coxiella species present in the two lineages of ticks may be different and may have co-evolved with them, thus driving different patterns of interactions between ticks and the pathogens that they can harbor or transmit to vertebrate hosts.

No abstract

Brazil is an important peanut producer, but despite its high production, there still needs to be an inoculant for the peanut crop. In addition, the use of microorganisms that promote plant growth (PGPM) is not common, and this crop is highly dependent on chemical fertilizers. An excellent alternative to reduce the use of fertilizers and chemical inputs in peanut crops while reducing the production cost and environmental impact is the use of PGPM. The objective of this study was to evaluate the effects of Azospirillum brasilense, Bacillus subtilis, Bradyrhizobium japonicum, and Trichoderma harzianum as single inoculants and co-inoculants on the growth promotion and productivity of peanuts in greenhouse and field conditions. In the greenhouse, the experiment was conducted with 12 treatments with six repetitions. In the field conditions, the experiment was conducted with five treatments with four repetitions. Both experiments were conducted in randomized blocks. In general, all the microorganisms evaluated in the present study promoted increases in root dry mass, shoot dry mass, phosphorus concentrations, and plant height in the greenhouse and under field conditions compared with the control. Interestingly, the mixtures of microorganisms inoculated in peanut plants did not promote greater plant growth and development compared with inoculations of the microorganisms separately. Specifically, in the field, the highest productivity was found for the inoculation of B. japonicum alone. The PGPM evaluated in the present study for peanut crops generally promoted some increases in productivity in greenhouse and field conditions.

De novo RNA-Seq assembly facilitates the study of transcriptomes of non-model, underutilized crops, enabling researchers to capture the maximum number of genes expressed in plant tissues. We were able to describe the expression profiling of the sweet passion fruit (Passiflora alata) in response to Xanthomonas axonopodis pv. passiflorae(Xap) infection. The crop is appreciated for the typical aroma and characteristic flavor of its fruits. However, yield is impaired by Xap, whose effects are exacerbated by high temperature and humidity. Initially, we provided the P. alata transcriptome assemblies which were shown to have high completeness, based on the expected gene content for a de novo transcriptome assembly. A total of 1,329 were completed genes and 96.6% of the orthologs conserved across Embryophytes were represented in the assembled transcriptome. Genes involved in pathogen recognition such as PRRs, R genes and genes related to the signaling cascade, coding for specific transcription factors and secondary metabolites, were found to be upregulated after infection. P. alata is known to be susceptible to Xap, thus we were interested in identifying possible susceptibility (S) genes. Interestingly, both characterized S genes in other plant species i.e., SWEET10 and LOB1were found to be upregulated in P. alata, suggesting that an effector-triggered susceptibility was achieved through the interaction between Xap and P. alata. Our qPCR results corroborate the role played by these genes, which could potentially be targets for genome editing in order to produce disease-resistant cultivars.

Sugarcane (Saccharum spp.) represents a crop of great economic importance, remarkably relevant in the food industry and energy supply chains from renewable sources. However, its conventional cultivation involves the intensive use of fertilizers, pesticides, and other agrochemical agents whose detrimental effects on the environment are notorious. Alternative systems, such as organic farming, have been presented as an environmentally friendly way of production. Still, the outcomes of different cropping systems on the microbiota associated with sugarcane—whose role in its health and growth is crucial—remain underexplored. Thus, we studied the rhizospheric microbiota of two adjacent sugarcane fields, which differ in terms of the type of farming system. For this, we used the sequencing of taxonomic markers of prokaryotes (gene 16S rRNA, subregions V3–V4) and fungi (Internal transcribed spacer 2) and evaluated the changes caused by the systems. Our results show a well-conserved microbiota composition among farming systems in the highest taxonomic ranks, such as phylum, class, and order. Also, both systems showed very similar alpha diversity indices and shared core taxa with growth-promoting capacities, such as bacteria from the Bacillus and Bradyrhizobium genera and the fungal genus Trichoderma. However, the composition at more specific levels denotes differences, such as the separation of the samples concerning beta diversity and the identification of 74 differentially abundant taxa between the systems. Of these, 60 were fungal taxa, indicating that this microbiota quota is more susceptible to changes caused by farming systems. The analysis of co-occurrence networks also showed the formation of peripheral sub-networks associated with the treatments—especially in fungi—and the presence of keystone taxa in terms of their ability to mediate relationships between other members of microbial communities. Considering that both crop fields used the same cultivar and had almost identical soil properties, we conclude that the observed findings are effects of the activities intrinsic to each system and can contribute to a better understanding of the effects of farming practices on the plant microbiome.