Dual-mode contrast agents (CAs) have great potential for improving diagnostics. However, the effectiveness of CAs is strictly related to both the solution adopted to merge the two agents into a single probe unit, and the ratio between the two agents. In this study, two dual-mode CAs for simultaneous magnetic resonance imaging (MRI) and ultrasound imaging (UI) were assessed. For this purpose, different densities of superparamagnetic iron oxide nanoparticles (SPIONs) were anchored to the external surface of polymer-shelled microbubbles (MBs) or were physically entrapped into the shell. In vitro static and dynamic experiments were carried out with a limited concentration of modified MBs (106 bubbles ml−1) by avoiding destruction during UI (performed at a peak pressure lower than 320 kPa) and by using a low-field MRI system (with a magnetic flux density equal to 0.25 T). Under these conditions, different imaging techniques, set-up parameters and SPION densities were used to achieve satisfactory detection of the CAs by using both UI and MRI. However, when the SPION density was increased, the MRI contrast improved, whereas the UI contrast worsened due to the reduced elasticity of the MB shell. For both UI and MRI, MBs with externally anchored SPIONs provided better performance than MBs with SPIONs entrapped into the shell. In particular, a SPION density of 29% with respect to the mass of the MBs was successfully tested.

In order to well distinguish different tissues of the human body by magnetic resonance imaging (MRI), it is of great importance to find procedures to improve the image contrast. In particular, a valuable feature is to image only specific parts of organs and/or tissues while ignoring all the others. Dedicated MRI sequences able to filter the 1 H nuclei signals based on the different longitudinal relaxation times (T1) of the tissues have been developed. Standard signal selection/attenuation sequences, such as the Short Time Inversion Recovery and Multiple Inversion Recovery, have the effect to zero the signal for a discrete number of T1 values. Parametrically Enabled Relaxation Filters with Double and multiple Inversion (PERFIDI) sequences act on a range of T1 values and behave as an electronic band-pass or high-pass or low-pass filters. PERFIDI filters are therefore primarily focused on the components that pass through, rather than on those that are blocked. These filters have been developed and tested by nuclear magnetic resonance relaxometry. Here, these sequences have been validated for MRI on phantom samples to mimic T1 distributions present in tissues. Preliminary applications show that PERFIDI filters can effectively work on a range of T1 values to give well contrasted images.

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