ObjectivesWe explored histological and transcriptomic profiles of paired synovial biopsies from rheumatoid arthritis (RA) patients, in order to assess homogeneity in synovial tissue at the individual level.MethodsSynovial biopsies were performed simultaneously in one small and one large joint per patient using needle-arthroscopy for the knee and ultrasound-guided biopsy for the hand or wrist. Synovium from individuals with osteoarthritis was used as controls. Paraffin-embedded samples were stained for CD3, CD20, and CD68. Total RNA was hybridized on high-density microarrays. TCRB variable sequences were obtained from synovial and blood RNA samples.ResultsTwenty paired biopsies from 10 RA patients with active disease were analyzed. Semi-quantification of histological markers showed a positive correlation for synovial hyperplasia, inflammatory infiltrates and CD3-positive T cells between pairs. Pairwise comparison of transcriptomic profiles showed similar expression of RA-related molecular pathways (TCR signaling, T cell costimulation and response to TNFα). T cells clonotypes were enriched in all but one joints compared to blood, regardless of the magnitude of T cell infiltration. Enriched clonotypes were shared between pairs (23–100%), but this was less the case in pairs of joints displaying weaker T cell signatures and more pronounced germinal center-like transcriptomic profiles.ConclusionCellular and molecular alterations in RA synovitis are similar between small and large joints from the same patient. Interindividual differences in magnitude of T cell infiltrates and distribution of enriched T cell clonotypes support the concept of distinct synovial pathotypes in RA that are associated with systemic versus local antigen-driven activation of T cells.

BackgroundRheumatoid Arthritis (RA) is a chronic and heterogenous condition characterized by inflammatory involvement of the synovial membrane in multiple joints. Synovial biopsies are used in research setting in order to identify diagnostic and theranostic markers. Many studies have shown a high degree of heterogeneity in histological and transcriptomic profiles between patients.ObjectivesWe wanted to explore histological and transcriptomic profile of synovial biopsies across pairs of joints in the same patients to assess heterogeneity at the individual level.MethodsSynovial biopsies were performed simultaneously in one small and one large joint per patient using needle-arthroscopy for the knee and US-guided needle biopsy for the hand or foot. Synovium from individuals with osteoarthritis (OA) were used as control. Paraffine-embedded samples were stained for CD3, CD 20, CD 68 and CD138. Whole-tissue RNA was extracted and hybridized on GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix). The samples were tested for RNA integrity by Bioanalyzer (Agilent). Normalization and statistical analyzes were performed on Genespring. Pathway analysis were performed using DAVID.Results10 RA patients were included (females: 10/10, ACPA/RF positivity: 8/10, mean age (± SEM): 54.4 (± 4.4) years, mean disease duration (± SEM): 13.3 (± 3.7) years, mean DAS28CRP (± SEM): 5.01 (± 0,34), mean HAQ (± SEM): 1.7 (± 0.28)).Quantification of histological markers did not show differences in population of macrophages, plasmocytes, T and B Cells, across pairs of joints.After correction for multiple comparisons, no transcripts were differentially expressed between large and small joints. Similarly, we did not find any significant difference in the expression of transcripts involved in pathways (TCR-activation and cell-division) specifically overexpressed in RA compared to OA synovial tissue.In order to increase our ability to observe pair-wise differences in gene expression profiles, we studied correlations between transcripts significantly overexpressed in RA compared to OA joints with a fold change > 2 (n = 581) and clinical or biological markers of disease activity (DAS28-CRP, CRP, Physician Global Assessment of disease activity). Similar patterns of correlations indicated that disease activity was not driven by different pathways in small versus large joints.ConclusionThis study is an important methodological milestone in the field of synovial biopsies, as it indicates that cellular and molecular alterations occurring in RA synovitis are similar across small and large joints from the same patient. Hence, biopsy of a single joint is representative and can be used to explore pathogenic processes or potential biomarkers in RA.AcknowledgementThis work was funded in part by grants from Cap48 (RTBF), Fonds National de la Recherche Scientifique (FNRS, Communauté française de Belgique) and Fondation Saint-Luc.Disclosure of InterestsClément Triaille: None declared, Louise Vansteenkiste: None declared, Laurent Meric de Bellefon: None declared, Ad...