The Escherichia coli Fur protein, with its iron(II) cofactor, represses iron assimilation and manganese superoxide dismutase (MnSOD) genes, thus coupling iron metabolism to protection against oxygen toxicity. Iron assimilation is triggered by iron starvation in wild-type cells and is constitutive in fur mutants. We show that iron metabolism deregulation in fur mutants produces an iron overload, leading to oxidative stress and DNA damage including lethal and mutagenic lesions. fur recA mutants were not viable under aerobic conditions and died after a shift from anaerobiosis to aerobiosis. Reduction of the intracellular iron concentration by an iron chelator (ferrozine), by inhibition of ferric iron transport (tonB mutants), or by overexpression of the iron storage ferritin H-like (FTN) protein eliminated oxygen sensitivity. Hydroxyl radical scavengers dimethyl sulfoxide and thiourea also provided protection. Functional recombinational repair was necessary for protection, but SOS induction was not involved. Oxygen-dependent spontaneous mutagenesis was significantly increased in fur mutants. Similarly, SOD deficiency rendered sodA sodB recA mutants nonviable under aerobic conditions. Lethality was suppressed by tonB mutations but not by iron chelation or overexpression of FTN. Thus, superoxide-mediated iron reduction was responsible for oxygen sensitivity. Furthermore, overexpression of SOD partially protected fur recA mutants. We propose that a transient iron overload, which could potentially generate oxidative stress, occurs in wild-type cells on return to normal growth conditions following iron starvation, with the coupling between iron and MnSOD regulation helping the cells cope.

No abstract

Organizational health literacy is described as an organization-wide effort to transform organization and delivery of care and services to make it easier for people to navigate, understand, and use information and services to take care of their health. Several health literacy guides have been developed to assist healthcare organizations with this effort, but their content has not been systematically reviewed to understand the scope and practical implications of this transformation. The objective of this study was to review (1) theories and frameworks that inform the concept of organizational health literacy, (2) the attributes of organizational health literacy as described in the guides, (3) the evidence for the effectiveness of the guides, and (4) the barriers and facilitators to implementing organizational health literacy. Drawing on a metanarrative review method, 48 publications were reviewed, of which 15 dealt with the theories and operational frameworks, 20 presented health literacy guides, and 13 addressed guided implementation of organizational health literacy. Seven theories and 9 operational frameworks have been identified. Six health literacy dimensions and 9 quality-improvement characteristics were reviewed for each health literacy guide. Evidence about the effectiveness of health literacy guides is limited at this time, but experiences with the guides were positive. Thirteen key barriers (conceived also as facilitators) were identified. Further development of organizational health literacy requires a strong and a clear connection between its vision and operationalization as an implementation strategy to patient-centered care. For many organizations, becoming health literate will require multiple, simultaneous, and radical changes. Organizational health literacy has to make sense from clinical and financial perspectives in order for organizations to embark on such transformative journey.

A national survey of health risk perception among 1,503 Canadians was conducted in 2004. The current survey follows-up a previous national survey conducted in 1992 and documents changes in risk perception since that time and investigates new risk issues not previously examined. This article presents a description of the ratings of perceived risk of thirty specific hazards to the Canadian population, sources of information about health issues and risk, and confidence in these information sources. Of the specific hazards considered, behavioral risks such as cigarette smoking, obesity, and unprotected sex were seen to present the greatest risk to the health of Canadians. Hazards related to the social environment (e.g., homelessness, street crime, unemployment) were seen as posing moderately high health risks. Medical devices or therapies (e.g., prescription drugs, vaccines, laser eye surgery) tended to rank the lowest in terms of health risk. Women, older respondents, and those with less education reported risks as being higher than men, younger respondents, and those with more education respectively. Large geographical differences in risk perception were also observed. Participants described receiving "a lot" of information from the news media, medical doctors, and the Internet but reported the greatest amount of confidence in medical doctors, university scientists/scientific journals, and health brochures/pamphlets.

No abstract

No abstract

The purpose of this study was to examine the effect of three Therapeutic Touch treatments on the well-being of 20 persons with terminal cancer in palliative care. Participants in the experimental group (n = 10) received three noncontact Therapeutic Touch treatments, the duration of which varied between 15 to 20 minutes. Participants in the control group (n = 10) participated in three rest periods. Well-being was measured at preintervention time and immediately postintervention time using the Well-Being Scale, a visual analogue scale measuring pain, nausea, depression, anxiety, shortness of breath, activity, appetite, relaxation, and inner peace. The results of the study support the hypothesis that three noncontact Therapeutic Touch treatments increase sensation of well-being in persons with terminal cancer.

This study explored the experience of eight grandparents having a grandchild diagnosed with cancer. Semi-structured interviews, both with individuals and couples, were used to gain a better understanding of the meaning these grandparents give to this experience. A thematic analysis based on Giorgi's (1997) phenomenological method highlighted three central themes: "Living the worst experience", "Having to support: A crucial role for grandparents", "To feel supported to better carry on." The results of this study can contribute to enhancing the quality of care for grandparents affected by their grandchild's cancer.