Polyploid breeding is an effective tool to induce variability in the existing population, specifically in the crops having very narrow genetic base such as acid lime. Hence, an experiment was conducted to induce polyploidy in acid lime through colchicine treatment. Higher frequency of triploids were obtained in 250 μM colchicine treatment (27.27%), whereas combination of 3× (21.42%), 4× (13.33%) and aneuploid (6.67%) were induced at 500 μM colchicine treatment. Measurement of stomatal density and guard cell size of colchiploids revealed that the former one has negative relation with ploidy level and a positive relation was observed between guard cell size and ploidy level. Further, area and size of nuclei also had a positive relation with ploidy level. Hence, stomatal density, guard cell and nuclei size can be used effectively as morphological marker for preliminary polyploid screening. From the experiment, it can be concluded that 500 μM colchicine treatment of in vitro derived shoot tip explants of acid lime is optimum for creating maximum possible variability through polyploidization in the existing acid lime population.
Evaluation of fruit crops has been successfully utilized for studying the performance of varieties under different agro climatic regions time to time. In the present study cultivars were characterized on the basis of their physico-biochemical attributes. "Mallika" and "Neelgoa" were found superior in terms of fruit weight (321.87 g), size (12.55 cm, 8.13 cm), pulp weight (257.91 g) and pulp stone ratio (7.71) respectively. "Mallika" excelled in terms of sugar (20.82), while "Amrapali" in carotenoids (8.38 mg/100 g). Among them, Mallika (22.41• B) possessed the highest amount of total soluble solids while lowest amount in Langra (16.90• B) whereas maximum titrable. The study shows the potential of Amrapali in terms of its quality, being late can meet the demand for later period when no other cultivar is available.
Quality of fruit crop is an important parameter to decide the acceptability of the product. The present study consists of seventeen year old pear (Pyrus pyrifolia (Burm.) Nakai) trees subjected to seven treatments viz., GA3 (50 ppm,100 ppm), BR (0.5 ppm, 1.0 ppm), and GA3 + BR (50 ppm + 0.5 ppm and 100 ppm + 1 ppm) and water as control, sprayed thrice at 15 days intervals starting from petal fall stage. Each treatment was replicated thrice with one tree served as a treatment unit. The experiment was conducted in Randomized Block Design. The fruits treated with GA3 @ 50 ppm (T1) showed the highest fruit length (6.98 cm), breadth (6.81 cm), weight (175.9 g) and volume (171.16 cc). An improvement in terms of fruit quality was observed either alone or in combined application of GA3 and BR. The application of BR @ 1 ppm (T4) recorded the highest TSS (12.91°Brix) and lowest titrable acidity (0.42%) while the highest ascorbic acid content (6.95 mg / 100 g) and non reducing sugar (0.44%) was estimated under GA3 @ 100 ppm + BR @ 1 ppm (T6). Total sugar (7.88%) and reducing sugar (7.45%) was observed highest in GA3 @ 50 ppm + BR @ 0.5 ppm (T5). Based on this research combined application of GA3 + BR had a positive effect and therefore can be recommended for spray on pear in order to obtain higher yield and better quality.
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