A technique for the controlled incubation of largemouth bass eggs (Micropterus salmonides) has been demonstrated at the Warmwater Fish Cultural Development Center, Marion, Alabama [9]. During the search for an acceptable incubation method, occasional high mortality of embryos was encountered and attributed to disease. Since the brood fish from which the eggs were obtained had a history of chronic hemorrhagic septicemia caused by Aeromonas liquefaciens [8, 10], prophylaxis of the eggs by disinfection (a common practice in trout culture [3, 4, 5, 6 ]) seemed applicable.
MATERIALS AND METHODS'The objective of this study was to determine which of the six readily available chemicals I previously used for trout egg disinfection would be best for protecting bass eggs against infection by A eromonas liquefaeiens.The two strains of A. liquefaeiens used to infect the bass eggs were from Auburn, and Marion, Alabama. Both were initially isolated from largemouth bass, and were maintained on trypticase soy agar (TSA) or in nutrient broth (NB).
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